食品科学 ›› 2004, Vol. 25 ›› Issue (6): 153-156.

• 分析检测 • 上一篇    下一篇

维生素B1、B2含量测定方法比较

 郭亚东, 马银海, 李燕华, 李璠   

  1. 昆明医学院药学院,昆明师范高等专科学校
  • 出版日期:2004-06-15 发布日期:2011-10-24

Comparison in Determination Methods of Vitamin B1,B2

 GUO  Ya-Dong, MA  Yin-Hai, LI  Yan-Hua, LI  Fan   

  1. 1.Kunming Medical College;2.Kunming Normal College
  • Online:2004-06-15 Published:2011-10-24

摘要: 目的:比较维生素B1、B2含量测定方法。方法:采用紫外分光光度法中三种不同方法测定维生素B1、B2含量。结果:1、按E值测标示量维生素B1:98.3±2.51,维生素B2:94.8±0.308,复合维生素B片中维生素B1:102.1±1.70,维生素B1平均回收率为100.4%,RSD为2.13%(n=6),维生素B2平均回收率为94.9%,RSD为6.5%(n=6)。2、标准曲线法维生素B1、B2浓度分别在3~18、1~8μg/ml范围内,均呈良好线性关系,标示量分别为:102.9±0.661,98.4±0.152。3、双波长法测维生素B1标示量:94.2±1.97。结论:三种方法用于维生素B1、B2含量测定,操作简便、快速、准确,精密度好。

关键词: 紫外分光光度法, 维生素B1, 维生素B2, 复合维生素B片

Abstract: Objective: To compare the determination methods of VB1, VB2. Methods: the three different methods of UV fordetermining VB1, VB2 are adopted. Results: According to the value of E, the contents were 98.3±2.51 for VB1 and 94.8±0.308 for VB2, the VB1 content of compound vitamin B tablets was 102.1±1.70. The average recovery for VB1 and VB2 were100.4 and 94.9 respectively. According calibration curve method: the concentration of VB1 and VB2 were 3~18 and 1~8μg/ml, showed good linear relationship. The results were 102.9±0.661 for VB1 and 98.4±0.152 for VB2. The dual wavelengthspectrophotometery: the content of VB1 was 04.8±1.97. Conclutions: The three methods could be used to the determination of VB1,VB2.They are simple, rapid, accurate, the precision are very good.

Key words: UV, vitamin B1, vitamin B2, compound vitamin B tablets