食品科学 ›› 2004, Vol. 25 ›› Issue (6): 116-119.

• 工艺技术 • 上一篇    下一篇

不同的脱蛋白方法用于螺旋藻多糖提取工艺的研究

 马丽, 覃小林, 刘雄民, 邓润祯   

  1. 广西大学工业测试实验中心,广西出入境检验检疫局
  • 出版日期:2004-06-15 发布日期:2011-10-24

Different De-protein Processes for Extraction of Polysaccharide from Spirulina Platensis

 MA  Li, QIN  Xiao-Lin, LIU  Xiong-Min, DENG  Run-Zhen   

  1. 1.The Industrial Testing and Experimental Center of Guangxi University;2.Guangxi Entry-Exit Inspection and Quarantine Bureau Technology Center
  • Online:2004-06-15 Published:2011-10-24

摘要: 对比了两种不同的脱蛋白方法(酶+Sevage法和三氯醋酸法)在螺旋藻多糖提取工艺中的应用。研究结果表明,不同的除蛋白方法不仅影响蛋白质的去除率和多糖的得率,而且所得到的螺旋藻多糖在组分、分子大小及单糖组成上存在较大差异。

关键词: 螺旋藻多糖, 脱蛋白, 纯化

Abstract: Two different de-protein processes (Enzymolysis+Sevage’s method and Trichloroacetic acid method (TCA )) wereproposed to improve the traditional process for extraction of polysaccharide from Spirulina Platensis. The production rate of crudepolysaccharides (ESPE, TSPS, SPS), the contents of polysaccharides and the remained protein ratio in the crude polysaccharides,etc, were compared in this paper. ESPS and TSPS were further purified by loading on DE-52 and Sephadex - 100 . Twopolysaccharide fractions of ESPS and three fractions of TSPS were obtained. The molecular weights of these fractions were 96,200、85800, 64300, 37400, 17400 respectively. The different efficacy of different molecular weight polysaccharides shouldbe studied further.

Key words: Spirulina Platensis polysaccharide, de-protein, purification