食品科学 ›› 2004, Vol. 25 ›› Issue (6): 113-116.

• 工艺技术 • 上一篇    下一篇

酶法水解米渣蛋白制备大米小分子肽

 顿新鹏, 陈正望   

  1. 华中科技大学生命科学与技术学院
  • 出版日期:2004-06-15 发布日期:2011-10-24

Enzyme Hydrolysis Preparation of Rice Small Polypeptides from Rice Residue

 DUN Xin-Peng , CHEN  Zheng-Wang   

  1. College of Life Science and Technology,Huazhong Sci-technology University
  • Online:2004-06-15 Published:2011-10-24

摘要: 实验用五种蛋白水解酶水解米渣蛋白,结果表明胰酶的作用效果最好。在底物浓度为8%、胰酶与底物比值为6%、温度为50℃、时间为6h的水解条件下,蛋白质提取率为83.02%,水解度为25.03%。经高效液相色谱凝胶过滤法测定,水解产物的分子量均小于5000Da,其中分子量小于1000Da的占52.30%。

关键词: 米渣蛋白, 水解, 提取率, 水解度, 分子量

Abstract: Five proteinases were used to hydrolyze rice residue in the research. The result showed that the effect of trypsinwas the best. When the substrate concentration 8%, trypsin -substrate ratio 6%, temperature 50℃ and the hydrolytic time6h, The rate of extraction was 83.02% and the degree of hydrolysis 25.03%.The molecular mass of hydrolysate was determinedby HPLC gel filtration. All the molecular mass was below 5000Da. Among them the molecular mass below 1000Da reached52.30%.

Key words: rice residue, hydrolyzation, rate of extraction, degree of hydrolysis, molecular mass