食品科学 ›› 2004, Vol. 25 ›› Issue (7): 125-128.

• 分析检测 • 上一篇    下一篇

混合植物甾醇标样的精制及其在分析中的应用

 陈茂彬, 吴谋成   

  1. 华中农业大学食品科技学院,湖北工学院生物工程系
  • 出版日期:2004-07-15 发布日期:2011-10-24

Preparation of Mixed Plant Sterol Standard Sample and Its Application in Analysis

 CHEN  Mao-Bin, WU  Mou-Cheng   

  1. 1.College of Food Science and Technology,Huazhong Agricultural University;2.Department of Bioengineering,Hubei Polytechnic University
  • Online:2004-07-15 Published:2011-10-24

摘要: 研究了重结晶法精制混合植物甾醇标准品的最佳溶剂和结晶操作条件,经过三次重结晶,植物甾醇总含量>99%,总收率>55%。探索了混合植物甾醇标准品在植物甾醇气相色谱定量分析中的应用,方法具有方便快捷、费用低、实用性强等特点。

关键词: 植物甾醇, 标样, 重结晶, 气相色谱

Abstract: The preparation and purification of mixed plant sterol standard sample by a recrystallization method were studied.Best solvents and operate conditions were found .The experimental results showed that the total content of plant sterol washigher than 99% and the total yield was higher than 55% after the third recrystallization operation. The applications of mixedplant sterol standard sample in quantitative analysis by gas chromatography were studied. These methods were found convenient,short-cut, cheap and practical.

Key words: plant sterol, standard, recrystallization, gas chromatography