食品科学 ›› 2004, Vol. 25 ›› Issue (7): 47-51.

• 基础研究 • 上一篇    下一篇

兔骨骼肌肌球蛋白分子形成凝胶过程的负染观察

 黄鸿兵, 徐幸莲, 周光宏   

  1. 南京农业大学农业部农畜产品加工与质量控制重点开放实验室
  • 出版日期:2004-07-15 发布日期:2011-10-24

Observation of Gel Formation from Rabbit Striated Muscle Myosin Molecules

 HUANG  Hong-Bing, XU  Xing-Lian, ZHOU  Guang-Hong   

  1. Key Laboratory of Agricultural and Animal Products Processing and Quality Control,Ministry of Agriculture,Nanjing Agriculture University
  • Online:2004-07-15 Published:2011-10-24

摘要: 肌球蛋白凝胶形成是肉制品粘结的基础。试验通过电子显微镜对兔骨骼肌肌球蛋白经不同温度加热处理后形态变化的负染观察,以探寻凝胶形成的过程。肌球蛋白经加热后,在加热温度低于40℃时,变性凝聚程度较低;而在高于40℃时,蛋白质变性凝聚成聚合体。并且,随着温度的升高,聚合程度加深;在60℃时分子已经形成直径大于500nm的聚合体。头头连接似乎是主要的连接方式。而尾尾相连在本试验中没有出现。

关键词: 兔, 骨骼肌, 肌球蛋白, 分子, 凝胶, 电子显微镜, 加热

Abstract: Heat induced gels of myosin formed and basis of the adhesiveness in meat products.In our experiment,the mannerin which a gel network was formed from rabbit striated muscle myosin molecules was investigated when myosin solution washeated at different temperatures between 30 and 60℃.The aggregates were examined by transmission electron microscopy afternegative staining.Molecules after heated at lower temperature(<40℃)denatured and aggregated only slightly;and when heatedat higher temperature(>40℃), molecules began to denature and aggregate shprply.Following the higher temperature, it formedfrom dimers to large aggregations (>500nm). It seemed that head-head likage predominated the aggregation, whereas tail-tail linkagedid not appear in the experiment.

Key words: rabbit, striated muscle, myosin, molecle, Gel, electron microscopy, heating