食品科学 ›› 2004, Vol. 25 ›› Issue (7): 36-42.

• 基础研究 • 上一篇    下一篇

DPPH自由基比色法测定番石榴叶提取物抗氧化活性的研究(英文)

 Venant Nihorimbere, 钱和, 汪何雅, 陈军杰, 梁清蓉   

  1. 江南大学食品学院教育部功能食品工程研究中心
  • 出版日期:2004-07-15 发布日期:2011-10-24

Evaluation of Antioxidant Activity ofPsidium guajava Linn (Guava) Leaf Extracts by Means of DPPH Free Radical

 Venant  Nihorimbere, QIAN  He, WANG  He-Ya, CHEN  Jun-Jie, LIANG  Qing-Rong   

  1. Food College of Southern Yountze University, National FunctionalFood Engineering
  • Online:2004-07-15 Published:2011-10-24

摘要: 本文用2,2-联苯-1-三硝基苯肼(DPPH•)比色法测定了番石榴叶(PsidiumguajavaL.)的抗氧化活性,并使用了三个不同参数抑制率、EC50和AE来评价其抗氧化活性。结果表明,浓度越高,番石榴叶提取物清除自由基的能力越强,其中AE是表示抗氧化活性的最有效的能数。本研究表明番石榴叶提取物是一种有效的、有开发价值的天然抗氧化剂。

关键词: 多酚, 抗氧化剂, 去自由基活性, DPPH&bull, 自由基, 比色法, 番石榴

Abstract: Antioxidant activity of Guava leaf was determined at ambient temperature by means of a 2,2-diphenyl-1-picrylhydryzyl (DPPH•) colorimetry with detection scheme at 515 nm. The activity was evaluated by the decrease inabsorbance as the result of DPPH• color change from purple to yellow. The higher the sample concentration used, the higherthe free radical-scavenging effect. The results showed that ascorbic acid was substantial powerful antioxidant than theextracts from guava leaf. The parameter EC50 and the time needed to reach the steady state to EC50 concentration (TEC50) affectedthe antiradical capacity of the sample. The antiradical efficiency (AE) has been shown to be a more adequate parameter toselect antioxidants than the widely used EC50. This study revealed that guava leaf extracts are an effective potential source ofnatural antioxidants.

Key words: antioxidant capacity, radical-scavenging activity, DPPH free radical, colorimetry, guava, polyphenols