食品科学 ›› 2004, Vol. 25 ›› Issue (7): 28-31.

• 基础研究 • 上一篇    下一篇

豆渣细胞壁中伸展蛋白纯化的研究

 刘晶晶, 邓泽元, 于化泓   

  1. 南昌大学食品科学教育部重点实验室
  • 出版日期:2004-07-15 发布日期:2011-10-24

Study on Extensin Purification from the Cell Wall of Soybean Dreg

 LIU  Jing-Jing, DENG  Ze-Yuan, YU  Hua-Hong   

  1. The Key Laboratory of Food Science of MOE,Nanchang University
  • Online:2004-07-15 Published:2011-10-24

摘要: 本文主要研究了通过CM-纤维素柱和SephadexG-75柱色谱纯化豆渣细胞壁中的伸展蛋白。用CM-纤维素柱色谱纯化豆渣细胞壁蛋白时,得到3个组分;对某个组分进一步采用SephadexG-75柱色谱纯化。并对这些组分进行氨基酸含量及分子量测定,再根据各组分的氨基酸含量及其分子量来初步鉴定属于伸展蛋白的组分。

关键词: 豆渣, 伸展蛋白, 纯化

Abstract: The extensins extracted from the cell wall of soybean dregs were purified by CM-cellulose and Sephadex G-75chromatography. Three components were attained from the cell wall proteins of soybean dregs by CM-cellulose chromatography.The amount of amino acid and molecular mass of these components were determined through Amino Acid Analysis Instrumentand SDS-PAGE, respectively. The compound belonging to extensin was identified according to the amount of amino acid andmolecular mass of these components obtained.

Key words: soybean dregs, extensin, purification