马尾松树皮提取物的抗氧化性初步研究

食品科学 ›› 2004, Vol. 25 ›› Issue (9): 179-183.

马尾松树皮提取物的抗氧化性初步研究

崔映宇, 谢衡, 赖斐, 王金发

教育部基因工程重点实验室中山大学生科院，广东嵩珍营养源研究所

出版日期:2004-09-15 发布日期:2011-10-24

Preliminary Study on Antioxidative Activities of Pinus massoniana Bark Extract (PMBE)

CUI Ying-Yu, XIE Heng, LAI Fei, WANG Jin-Fa

1.Key Laboratory of Gene Engineering of Ministry of Education, College of Life Sciences, Sun Yat-sen University;2.Institute of Songzhen Nutritional Resource

Online:2004-09-15 Published:2011-10-24

摘要/Abstract

摘要： 目的:研究马尾松树皮提取物(Pinus massoniana bark extract, PMBE)在体外不同介质中的抗氧化能力和清除活性氧自由基能力,测定不同浓度PMBE对体外培养的人体8种癌细胞株的作用。方法:用化学比色法测定PMBE体外的抗氧化和清除活性氧能力,并与天然抗氧化剂VC和VE进行比较;用四甲基偶氮唑蓝比色法(measurement oftrititaed thymidine incorporation, MTT)测定PMBE对不同癌细胞株的体外作用。结果:PMBE在体外生理盐水条件下具有较强抗氧化和清除活性氧能力,其抗氧化能力稍弱于VC,约为VE的3.5倍,对不同癌细胞株的抑制浓度和抑制程度不同。结论: PMBE是一种天然抗氧化剂和活性氧清除剂,具有抑癌潜能。

关键词: 马尾松树皮提取物, 抗氧化剂, 活性氧, 人癌细胞株

Abstract: Objective: To investigate the competence of total antioxidation and scavenging reacticve oxygen radicals of Pinusmassoniana bark extract (PMBE) in different media and its effect on 8 human cancer cell lines cultured in vitro. Methods: Chemicalcolorimetry and measurement of tritiated thymidine incorporation (MTT) assay were applied to study the total antioxidationcompetence and reacticve oxygen radical-scavenging competence of PMBE in different media and its effect on human cancer celllines, respectively. Its antioxidative and reactive oxygen radical- scavenging competence was compared with that of naturalantioxidants vitamin C or vitamin E through chemical colorimetry. Results: PMBE owned higher competence of total antioxidationand scavenging reacticve oxygen radicals in normal saline in vitro, just a little less than VC and nearly 3.5 times as much as VE.Different inhibition degrees to different cancer cell lines with different concentration PMBE solutions were shown, respectively.Conclusion: PMBE would be a natural antioxidant and reactive oxygen radical scavenger, probably a functional potential forinhibiting cancer and could be applied in R&D of anticancer drug.

Key words: Pinus massoniana bark extract, antioxidant, reactive oxygen species, human cancer cell lines

崔映宇, 谢衡, 赖斐, 王金发. 马尾松树皮提取物的抗氧化性初步研究[J]. 食品科学, 2004, 25(9): 179-183.

CUI Ying-Yu, XIE Heng, LAI Fei, WANG Jin-Fa. Preliminary Study on Antioxidative Activities of Pinus massoniana Bark Extract (PMBE)[J]. FOOD SCIENCE, 2004, 25(9): 179-183.

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