食品科学 ›› 2004, Vol. 25 ›› Issue (9): 166-169.

• 营养卫生 • 上一篇    下一篇

茶多酚对染镍细胞脂质过氧化及DNA损伤的影响

 张敬, 张军, 石红军, 李卓权, 郭超, 郭俊生   

  1. 同济大学生命科学与技术学院,同济大学医学院生物教研室,第二军医大学海医系军队卫生教研室
  • 出版日期:2004-09-15 发布日期:2011-10-24

Protective Effects of Tea Polyphenols on Ni2O3-induced Lipid Peroxidation in Mice Alveolar Macrophage and DNA Strand Breakage of Human Lung Fibroblasts Cell

 ZHANG  Jing, ZHANG  Jun, SHI  Hong-Jun, LI  Zhuo-Quan, GUO  Chao, GUO  Jun-Sheng   

  1. 1.College of Life Science and Technology Tongji University;2.Department ofMilitary Hygiene, Faculty of Navy Medicine, Second Military Medical University;3. College of Medical Science, Biological Teaching Research Room, Tongji University
  • Online:2004-09-15 Published:2011-10-24

摘要: 采用体外细胞培养方法,观察茶多酚对染镍(Ni2O3,5mg/L)大鼠肺泡巨噬细胞膜脂质过氧化产物丙二醛(MDA)及超氧化物歧化酶(SOD)活性的影响。并用单细胞凝胶电泳技术(Single Cell Gel Electrophoresis, SCGE)检测三氧化二镍对人肺成纤维细胞(HLF)的DNA损伤作用以及茶多酚的保护作用。结果显示在体外染镍大鼠肺泡巨噬细胞培养过程中加入不同浓度茶多酚(125,250和500mg/L)可提高肺泡巨噬细胞内SOD活性,且能显著抑制MDA生成量。镍处理的HLF细胞DNA损伤程度高于正常对照组(p<0.01);而Ni2O3+ 茶多酚(TP,250mg/L)处理组DNA损伤程度低于Ni组(p<0.01)。镍可增加细胞脂质过氧化,茶多酚具有拮抗镍导致细胞毒性的作用,可能与其抗氧化功能有关。茶多酚还可以拮抗镍诱导的人肺成纤维细胞DNA的损伤。

关键词: 茶多酚, 三氧化二镍, 丙二醛, 超氧化物歧化酶, DNA损伤, 单细胞凝胶电泳

Abstract: To study the effects of tea polyphenols (TP) on the injury of mice alveolar macrophages treated with Ni2O3, the alveolarmacrophages were cultured in vitro with exposure to Ni2O3(5mg/L). Meanwhile, TP(125, 250 and 500mg/L)were added in themedium, respectively. The detection of malondialdehyde and the activity of antioxidant enzyme in alveolar macrophages werecarried out. The protective effects of TP against DNA damage of human lung fibroblasts (HLF) cells induced by Ni2O3 were alsoinvestigated. HLF cells were cultured in vitro and treated with Ni2O3(5mg/L) and Ni2O3+TP(250mg/L), respectively. The singlecell gel electrophoresis (SCGE) was used to detect the DNA breaks. The results showed that TP could increase the activity ofsuperoxide dismutase in mice alveolar macrophages in a dose-dependent manner and inhibit significantly the production ofmalondialdehyde at dose of 250mg /L and 500mg/L respectively in comparison with Ni2O3 group(p<0.05). The level of DNAbreaks induced by Ni2O3 was higher than that of the control group (p<0.01). But the level of DNA breaks induced by Ni2O3+TP was lower than that of the Ni2O3 treated group (p<0.01). All results demonstrated that TP could prevent cells from DNAdamage induced by Ni2O3.

Key words: tea polyphenols, Ni2O3, MDA, SOD, DNA damage, SCGE