食品科学 ›› 2004, Vol. 25 ›› Issue (9): 150-152.

• 分析检测 • 上一篇    下一篇

木瓜中齐墩果酸的分离提取及含量测定

 王文平, 周文美   

  1. 贵州工业大学化学与生物工程学院
  • 出版日期:2004-09-15 发布日期:2011-10-24

Extraction and Quantitative Determination of Oleanolic Acid in the Chinese Quince

 WANG  Wen-Ping, ZHOU  Wen-Mei   

  1. Chemistry and Biology Engineering College, Guizhou University of Technology
  • Online:2004-09-15 Published:2011-10-24

摘要: 本文对木瓜中的齐墩果酸进行了分离提取及含量测定研究。以氯仿为溶剂,热回流浸提,采用薄层层析法(TLC)对木瓜中的齐墩果酸进行定性分析,用高效液相色谱法(HPLC)进行含量测定,得到木瓜中的齐墩果酸的平均含量为0.0831%。该法准确度高,结果稳定。

关键词: 木瓜, 齐墩果酸, 薄层层析法, 高效液相色谱法

Abstract: This paper studied the isolation and extraction methods of oleanolic acid in the Chinese quince. Chloroform wasused to extract oleanolic acid by hot refluxing, and thin-layer chromatography(TLC) was adppted to conduct qualitativeanalysis. High performance liquid chromatography(HPLC) was used to determine oleanolic acid content. The results showedthat the average content of oleanolic acid was 0.0831% in the Chinese quince. This assay method was precise and reliable.

Key words: Chinese quince, oleanolic acid, TLC, HPLC