食品科学 ›› 2004, Vol. 25 ›› Issue (12): 47-50.

• 基础研究 • 上一篇    下一篇

米糠蛋白类阿片拮抗肽的分离纯化

 陈季旺, 陶冠军, 姚惠源   

  1. 武汉工业学院食品学院,江南大学分析测试中心,江南大学食品学院
  • 出版日期:2005-12-15 发布日期:2011-10-24

Isolation and Purification of Opioid Antigonist Peptides from Rice Bran Soluble Protein

 CHEN  Ji-Wang, TAO  Guan-Jun, YAO  Hui-Yuan   

  1. 1.Food School of Wuhan Polytechnic Unversity;2.Analytical Center of Southern Yangtz Unversity;3.Food School of Southern Yangtz Unversity
  • Online:2005-12-15 Published:2011-10-24

摘要: 采用体积排阻色谱和反相高效液相(RP-HPLC)对米糠蛋白酶解物进行分离纯化,得到一种具有较高活性的类阿片拮抗肽,体积排阻高效液相色谱(SE-HPLC)测定它的相对分子质量为546Da。

关键词: 类阿片拮抗肽, 分离纯化

Abstract: The opioid antigonist peptide was extracted from enzymatic hydrolysates of rice bran soluble protein isolated and purified by using SE-HPLC chromatography and RP-HPLC. The results from SE-HPLC indicated that the molecular weight of opioid antigonist peptide was 546Da.

Key words: opioid antagonist peptide, isolation and purification