食品科学 ›› 2004, Vol. 25 ›› Issue (10): 267-269.

• 分析检测 • 上一篇    下一篇

荞麦籽油脂肪酸的气相色谱/质谱法分析

 范铮, 宋庆宝, 强根荣, 孙培龙, 朱晓雨, 陈丹霞   

  1. 浙江工业大学生物与环境学院,浙江省出入境检验检疫局
  • 出版日期:2004-10-15 发布日期:2011-10-24

GC/MS Analysis of Fatty Acid in Fagopyrum Esculentum Seed Oil

 FAN  Zheng, SONG  Qing-Bao, QIANG  Gen-Rong, SUN  Pei-Long, ZHU  Xiao-Yu, CHEN  Dan-Xia   

  1. 1.College of Biology and Environment, Zhejiang University of Technology;2.Zhejiang Entry-Exit Inspection and Quarantine Bureau
  • Online:2004-10-15 Published:2011-10-24

摘要: 采用石油醚抽提荞麦籽,然后将油样进行皂化、甲酯化,以毛细管色谱-质谱联用法测定荞麦籽油中的组分,从分离出的50个峰中确认了23种组分,所鉴定的组分占总峰面积的96.95%。其中脂肪酸中主要成分含量:C18:2≥32.91%,C18:1≥24.73%,C16:0≥20.04%。奇数碳原子脂肪酸、鲨烯、23s-甲基胆甾醇、5-烯-3β-豆甾醇、5,24-二烯,3-豆甾醇、12-烯-3-醇-齐墩果、12-烯-3-醇-熊果等未见报道。

关键词: 荞麦, 脂肪酸, 化学组成, 色谱-质谱法

Abstract: In this paper, Fagopyrum esculentum seed oil was extracted with petroleum ether. Hydrolyzed and esterified withpotassium hydroxide and methanol. After saponification and esterification the oil was determined by capillary GC/MS. Morethan 50 peaks were separated and twenty-three compounds representing 96.95% of the oil were identified. C18:2 & C18:1 &C16:0 were chief components, and their relative contents exceeds 32.91% and 24.73% and 20.04%.There are no reported aboutodd number carbon atomy fatty acid, 2, 6, 10, 14, 18, 22-Tetracosahexaene, 23S-Methylcholesterol, Stigmast-5-en-3-ol, Stigmast-5, 24-dien-3-ol, Olean-12-en-3-ol, Urs-12-en-3-ol.

Key words:  Fagopyrum esculentum, fatty acid, chemical constituents, GC/MS