食品科学 ›› 2004, Vol. 25 ›› Issue (10): 247-250.

• 分析检测 • 上一篇    下一篇

鳗鱼中恩诺沙星残留量的酶联免疫检测方法

 郑晶, 黄晓蓉, 李耀平, 李小晶, 林杰   

  1. 福建出入境检验检疫局
  • 出版日期:2004-10-15 发布日期:2011-10-24

Determination of Remained Enrofloxacin in Eel by ELISA

 ZHENG  Jing, HUANG  Xiao-Rong, LI  Yao-Ping, LI  Xiao-Jing, LIN  Jie   

  1. Fujian Province Entry-Exct Inspection and Quarantine Bureau
  • Online:2004-10-15 Published:2011-10-24

摘要: 运用酶联免疫分析方法测定鳗鱼中恩诺沙星残留。测试曲线线性范围为0.3~10μg/kg,最低检测限为3μg/kg。向样品中分别添加25、50、100μg/kg 3个浓度水平的恩诺沙星, 平均回收率分别为74.5%、76.6%和66.5%,批内变异系数为7.62%~14.31%,批间变异系数为6.03%~7.00%,灵敏度达到0.3μg/kg。特异性试验结果表明与喹诺酮类中的其它药物以及其它类抗生素均无交叉反应。

关键词: 鳗鱼, 恩诺沙星残留, 酶联免疫方法

Abstract: Remained Enrofloxacin in eel were determined by ELISA. The calibration curve should be virtually linear in the rangeof 0.3~10μg/kg. The limit of detection (LOD) is 3μg/kg. Adding Enrofloxacin to eel samples, the concentration is 25, 50 and100μg/kg separately. Average recovery rate is 74.5%, 76.6%, and 66.5% separately. Coefficient of variation in same batch is7.62%~14.31%. Coefficient of variation in different batch is 6.03%~7.00%. Sensitivity is 0.3μg/kg. The rusult of special testis as following: No cross-reactivities happened to other drugs.

Key words: eel, remained Enrofloxacin, ELISA