食品科学 ›› 2004, Vol. 25 ›› Issue (10): 211-214.

• 分析检测 • 上一篇    下一篇

不同季节桑叶中1-脱氧野尻霉素(DNJ)含量的测定

 欧阳臻, 陈钧   

  1. 江苏大学生物与环境工程学院制药工程系
  • 出版日期:2004-10-15 发布日期:2011-10-24

Determination of 1-deoxynojirimycin in Morus alba L. Leaves in Different Seasons

 OU Yang-Zhen , CHEN  Jun   

  1. School of Biological and Environmental Engineering, Jiangsu University
  • Online:2004-10-15 Published:2011-10-24

摘要: 建立了柱前荧光衍生-高效液相色谱法测定桑叶中1-脱氧野尻霉素(DNJ)含量的方法。并对不同生长季节桑叶药材的DNJ含量进行测定。桑叶经0.05mol/L盐酸提取,在pH8.5的硼酸盐缓冲液条件下,用芴甲氧酰氯(FMOC-Cl)与DNJ反应生成荧光产物,然后用高效液相色谱-检测器测定。流动相为乙腈-0.1%醋酸(55:45,V/V);流速:1.0ml/min;柱温25℃;荧光检测器激发波长254nm,发射波长322nm。线性范围为0.567~34μg/ml(r=0.9999),平均回收率为97.2%。测定结果表明,桑叶中DNJ含量与生长季节、温度有关,七、八月份DNJ含量最高。该方法准确、灵敏度高,重现性好,可用于桑叶中DNJ的定量分析。

关键词: 桑叶, 1-脱氧野尻霉素(DNJ), 高效液相色谱法, 荧光衍生化

Abstract: A high performance liquid chromatographic method for the determination of DNJ in Morus alba L. leaves withfluorimetric detection after precolumn derivatization with 9-fluorenylmethyl chlorformate (FMOC-Cl) was established. Thecontents of DNJ in Morus alba L. collected within different vegetative periods were determined. DNJ in Morus alba L. leaveswas extracted with 0.05mol/L HCI, derivatized with FMOC-Cl, and analyzed by high performance liquid chromatographyequipped with fluorescence detector. Mobile phase consisted of acetonitrile -0.1% aqueous acetic acid(55:45,V/V)with aflow rate of 1.0ml/min. The calibration curve was linear over the concentration range from 0.567μg/ml to 34μg/ml, the correlationcoefficient was 0.9999. the average recovery was 97.2%, RSD was 1.97(n=6). The results showed that the content of DNJ inleaves of Morus alba L. is relative to the vegetative periods and temperature, the highest content of DNJ in leaves of morus albaL. collected at July and August. The method used this paper was reliable, accurate and stable. It can be used for determinationof the DNJ in leaves of Morus alba L.

Key words: Leaves of Morus alba L., 1-deoxynojirimycin, high performance liquid chromatography, precolumn derivatization