食品科学 ›› 2004, Vol. 25 ›› Issue (10): 187-191.

• 工艺技术 • 上一篇    下一篇

乌骨鸡深加工酶解处理条件的探索研究

 杨瑞学, 朱小艳   

  1. 北京同元制药厂
  • 出版日期:2004-10-15 发布日期:2011-10-24

Study on the Enzymolysis Condition of Black-boned Chicken

 YANG  Rui-Xue, ZHU  Xiao-Yan   

  1. Beijing Tongyuan Pharmaceutical Factory
  • Online:2004-10-15 Published:2011-10-24

摘要: 对乌骨鸡进行深加工,提高蛋白质的综合利用率,增加氨基酸尤其是游离氨基酸的含量,便于人体对其吸收利用。本论文阐述了研究国际上领先的酶制剂Flavourzyme风味蛋白酶水解乌骨鸡动物蛋白质的水解条件。特点是在原来采用传统炮制工艺提取乌骨鸡有效成分的基础上采用了现代生物工程-酶解技术,对乌骨鸡进行深度水解处理,比一般的和传统的酶解有更高的产率和更好的风味及口感。确定了正交分解试验方法,分别测定水解率,进行方案组合,确定最佳处理条件。结果表明,乌骨鸡肉经90℃,10min的加热预处理可促进酶解。酶解最佳条件为:pH=7.0,温度45℃,酶与底物浓度之比为0.5LAPU/g,底物浓度11%,时间3h。

关键词: 乌骨鸡, 酶解

Abstract: The deep processing of black-boned chicken is very important for the more efficient utility of the protein and toincrease the free amino acid content, so that it can be absorbed easily by the human body. This article described the internationaladvanced enzyme product, the hydrolytic condition of flavor proteinase for hydrolyzing animal protein of black-boned chicken.The feature is the use of the modern bioengineer - enzymolysis technique based on the traditional processing technique ofextracting the effective component of the black-boned chicken, by using the in-depth hydrolyzing process so that we can get biggerproductivity and flavor. We developed the orthogonal discomposing experiment method to measure the hydrolytic rate, tocombine the protocol and to determine the best processing condition. The result is that 10min heating pre-processing90℃ of temperature can promote the enzymolysis. The best enzymolysis condition is pH value 7.0, temperature 45℃ and theconcentration ratio of enzyme to substrate 0.5 and time of 3h.

Key words: black-boned chicken, enzymolysis