食品科学 ›› 2004, Vol. 25 ›› Issue (10): 119-122.

• 工艺技术 • 上一篇    下一篇

黄精含硒多糖的分离提取及含硒量分析技术研究

 汪兴平, 莫开菊, 周大寨, 单长海   

  1. 湖北民族学院生物科学与技术学院
  • 出版日期:2004-10-15 发布日期:2011-10-24

Extraction, Separation and Determination of Polysaccharides and Testing of Selenium(Se) in Polygonatum

 WANG  Xing-Ping, MO  Kai-Ju, ZHOU  Da-Zhai, DAN  Chang-Hai   

  1. School of Biological Science and Technology, Hubei Institute for Nationalities
  • Online:2004-10-15 Published:2011-10-24

摘要: 本文以富含硒元素恩施药材—黄精为原料,对黄精含硒多糖的分离提取技术进行了初步研究。实验采用水浸提、乙醇醇析提取、sevage脱蛋白、沉淀、真空冷冻干燥等方法研究黄精含硒多糖的提取工艺,并用蒽酮比色法和原子吸收法对多糖含量、硒含量进行了测验。结果表明:黄精水溶性含硒多糖最佳提取参数为:温度90℃、提取时间4h、料液比1:50、提取次数为2次,水溶性多糖含量为3.12%,硒的含量为0.5973μg/g。

关键词: 黄精多糖, 提取,

Abstract: This article used the medicinal material-Polygonatum which was rich in senelium element as experimental material,preliminarily studied the separating and extracting technology of Senelium polysaccharides.The experiments studied the extract-ing technology by the methods of water extracting, preciptation with alcohol, sevage deproteinizing, preciptation,vacuum freezingdrying and the content of polysaccharides and senelium were determined by anthrone colorimetry and atomic absorptionspectrometry.The optimum parameters to extract soluble selenium polysaccarides in Rhizoma Polygonati were as follows:temperature 90℃, extracting time 4h, the ratio of water to material 1:50, extracting times 2,the content of soluble seleniumpolysaccarides 3.12%.

Key words: polygonatum polysaccharides, extraction, selenium(Se)