食品科学 ›› 2004, Vol. 25 ›› Issue (10): 49-53.

• 基础研究 • 上一篇    下一篇

高产α-转移葡萄糖苷酶菌株的选育及其产酶条件研究

 毕金峰, 魏宝东, 李长彪   

  1. 中国农业科学院农产品加工研究所,沈阳农业大学食品学院
  • 出版日期:2004-10-15 发布日期:2011-10-24

Studies on Screening by Mutating Strain of Producing Alpha-Transglucosidase and Its Conditions for Producing Alpha-Transglucosidase

 BI  Jin-Feng, WEI  Bao-Dong, LI  Chang-Biao   

  1. 1.Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences;2.College of Food Science,Shenyang Agricultural University
  • Online:2004-10-15 Published:2011-10-24

摘要: α-转移葡萄糖苷酶是生产低聚异麦芽糖的关键酶。本文成功选育一株高产α-转移葡萄糖苷酶菌株ASP-2。并对该菌株进行了固态发酵条件优化,结果表明:麸皮和水的质量比为1:1,35℃培养48h时产酶量及酶活较高,ASP-2菌株生长的最适pH值为6.0,碳源和氮源不是该菌株在此培养基中产酶的必需元素,从降低生产成本的角度考虑,可以不用。

关键词: &alpha, -转移葡萄糖苷酶, 菌株, 选育, 产酶条件

Abstract: Alpha-transglucosidase is a critical enzyme of producing isomaltooligosaccharide. In this paper, strain ASP-2 ofproducing α-transglucosidase was obtained by screening experiments. The optimum conditions for fermentation in solid-statewere:bran:water(m/m) =1:1,culture temperature and duration were 35℃ and 48h respectively, the optimum pH value was 6.0.Carbon sources and nitrogen sources were not necessary nutrition for producing enzyme in this culture medium , so they needn’tbe added in the culture medium in order to reduce production cost.

Key words: α-transglucosidase, strain, screening, conditions for producing α-transglucosidase