食品科学 ›› 2004, Vol. 25 ›› Issue (10): 46-49.

• 基础研究 • 上一篇    下一篇

荧光假单胞菌AR4产2-酮基-D-葡萄糖酸发酵条件的研究

 孙文敬, 杨庆文, 黄惠英, 郭金权, 赵峰梅, 刘敬泽   

  1. 河北师范大学生命科学学院,山西省生物研究所,郑州市生物化工厂,
  • 出版日期:2004-10-15 发布日期:2011-10-24

Studies on the Fermentation Conditions of Pseudomonas fluorescens AR4 Producing 2-Keto-D-gluconic Acid

 SUN  Wen-Jing, YANG  Qing-Wen, HUANG  Hui-Ying, GUO  Jin-Quan, ZHAO  Feng-Mei, LIU  Jing-Ze   

  1. 1.College of Life Science, Hebei Normal University;2.Shanxi Institute ofBiology;3.Zhengzhou Biochemical Factory
  • Online:2004-10-15 Published:2011-10-24

摘要: 以淀粉水解糖为碳源,研究了不同氮源、碳氮比、金属离子、接种量以及环境因子(培养基起始pH值、通气量、温度)对抗噬菌体菌株荧光假单胞菌(Pseudomonas fluorescens)AR4产2-酮基-D-葡萄糖酸发酵的影响。结果表明,氮源、碳氮比、培养基的起始pH值、通气量及温度对该菌的产酸均有显著影响。在适宜培养条件下,该菌的摇瓶产酸及发酵转化率分别可达13.5%和90%左右。

关键词: 荧光假单胞菌AR4, 2-酮基-D-葡萄糖酸, 发酵, 抗噬菌体菌株

Abstract: Using saccharide from corn starch hydrolysate as a sole carbon source, the factors (nitrogen sources, carbon-nitrogenratio, metal ions, the volume of inoculation, initial pH value of the medium, aeration rate and temperature) affecting 2-keto-D-gluconic acid fermentation by bacteriophage-resistant mutant Pseudomonas fluorescens AR4 were investigated. The resultsshowed that the production of 2-keto-D-glluconic acid was significantly affected by nitrogen sources, carbon-nitrogen ratio,initial pH value of the medium, aeration rate and temperature. Under the suitable fermentation conditions, the acid yield and theconversion rate was about 13.5% and 90% respectively.

Key words: Pseudomonas fluorescens AR4, 2-keto-D-gluconic acid, fermentation, bacteriophage-resistant mutant