食品科学 ›› 2009, Vol. 30 ›› Issue (1 ): 119-121.doi: 10.7506/spkx1002-6630-200901028

• 基础研究 • 上一篇    下一篇

利用硅胶柱层析分离纯化平贝总碱的研究

翟爱华1,王丽杰2,张东杰1,鲁明明3   

  1. 1.黑龙江八一农垦大学食品学院 2.锦州医学院畜牧兽医学院 3.大连富生制药有限公司
  • 收稿日期:2008-04-06 出版日期:2009-01-01 发布日期:2010-12-29
  • 通讯作者: 翟爱华 E-mail:zhaiaihua@sina.com
  • 基金资助:

    黑龙江省科技攻关项目(GC04C50102)

Study on Separation and Purification of Total Alkaloids from Fritillaria ussuriensis Maxim by Silica Gel Column Chromatography

ZHAI Ai-hua1,WANG Li-jie2,ZHANG Dong-jie1,LU Ming-ming3   

  1. (1. College of Food Science, Heilongjiang August First Land Reclamation University, Daqing 163319, China;
    2. College of Animal Husbandry and Veterinary Medicine, Jinzhou Medical College, Jinzhou 121001, China;
    3. Dalian Fusheng Pharmaceutical Co. Ltd, Dalian 116600, China)
  • Received:2008-04-06 Online:2009-01-01 Published:2010-12-29
  • Contact: ZHAI Ai-hua1 E-mail:zhaiaihua@sina.com

摘要:

目的:对东北特产平贝母的生物碱进行分离纯化研究。方法:用硅胶柱层析法对平贝总碱进行分离纯化,固定相为薄层层析硅胶G,流动相为乙酸乙酯- 甲醇- 氨水(17:2:1)。用HPLC-ELSD 法对分离出的贝母素甲和贝母素乙的纯度进行测定。结果:分离出贝母素甲和贝母素乙,贝母素甲纯度为93.19%、贝母素乙纯度为92.09%。结论:采用的硅胶柱层析分离纯化可得到纯度较高的分离组分。

关键词: 平贝总碱, 硅胶柱层析, 分离纯化

Abstract:

Silica gel column chromatography was used to separate and purify total alkaloids from Fritillaria ussuriensis Maxim. The solid phase was thin layer chromatography silica gel G and the mobile phase was ethyl acetate-methanol-ammonia (17:2:1). The purities of the separated products, pemine and peinine were determined using HPLC-ELSD. They are 93.19% and 92.09%, respectively. In conclusion, the separation method of silica gel column chromatography applied in this experiment can be used to gain highly purified products.

Key words: total alkaloids from Fritillaria ussuriensis Maxim, silica gel column chromatography, separation and purification

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