一株降胆固醇乳酸菌的鉴定及其在模拟胃肠环境中抗性的研究

食品科学 ›› 2004, Vol. 25 ›› Issue (11): 281-284.

一株降胆固醇乳酸菌的鉴定及其在模拟胃肠环境中抗性的研究

张德珍, 潘道东, 戴传超

南京师范大学生命科学院，南京师范大学金陵女子学院食品科学与工程系

出版日期:2005-11-15 发布日期:2011-10-24

Studies on the Identification and Antagonistic Properties in the Imitative Gastroenteric Environment in a Cholesterol Reducing Lactic Acid Bacteria

ZHANG De-Zhen, PAN Dao-Dong, DAI Chuan-Chao

1.College of Life Science, Nanjing Normal University;2.Food Science and Tech-nology Department of Jinling Women’s College, Nanjing Normal University

Online:2005-11-15 Published:2011-10-24

摘要/Abstract

摘要： 鉴定一株有降胆固醇功能的益生菌LQ-12为乳酸乳球菌乳亚种。同时对该菌在模拟胃肠环境条件下的抗性及对肠道中致病性大肠杆菌、金黄色葡萄球菌的拮抗性和服用抗生素后的耐药性进行了研究。结果表明,LQ-12在pH1.5～4.5生活4h活菌数仍达106cfu/ml以上,在0.1%～0.3%胆汁盐条件下4h活菌数仍达106cfu/ml以上。同时对致病性大肠杆菌和金黄色葡萄球菌具有明显的抑制作用,且对氨苄青霉素、四环素、庆大霉素、严迪(主要成分为罗红霉素)和头孢拉定有耐药特性。可用作微生态制剂和酸奶发酵剂的优良菌种。

关键词: 乳酸菌, 抗性, 抑菌, 耐药

Abstract: The cholesterol-reducing probiotics——LQ-12 was identified as Lactococus.lactis subsp.lactis. As imitatinggastroenteric environments,such as low pH(1.5～4.5) and high bile salt(0.1%～0.4%),the antagonistic properties of LQ-12were studied.At the same time,the antimicrobial activities on pathogenic E.coli and S.aureus in the intestinal tract and antibiotictolerance of LQ-12 were also studied.The results indicated that LQ-12 survived at pH 1.5～4.5 after 4h culture and the livingbacterial number could reach 106cfu/ml;that living bacterial number were also above 106cfu/ml at the 0.1%～0.3% bile salt andeven survived in 0.4% after 6h culture;that antimicrobial activities to the pathogenic E.coli and S.aureus were obvious;andthat the tolerance to Ampicillin, Tetracycline, Gentamicin, Roxithromycin, Cefradine was power. So LQ-12 can be used as microbialpreparation and starter for yoghurt.

Key words: lactic acid bacteria, antagonistic property, antimicrobe, medicine tolerance

张德珍, 潘道东, 戴传超. 一株降胆固醇乳酸菌的鉴定及其在模拟胃肠环境中抗性的研究[J]. 食品科学, 2004, 25(11): 281-284.

ZHANG De-Zhen, PAN Dao-Dong, DAI Chuan-Chao. Studies on the Identification and Antagonistic Properties in the Imitative Gastroenteric Environment in a Cholesterol Reducing Lactic Acid Bacteria[J]. FOOD SCIENCE, 2004, 25(11): 281-284.

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