食品科学 ›› 2009, Vol. 30 ›› Issue (19 ): 200-202.doi: 10.7506/spkx1002-6630-200919045

• 生物工程 • 上一篇    下一篇

分子生物学方法区分鉴定五种常用葡萄酒活性干酵母

李 艳1,2,卢 君1,崔建东1,2,杨美景1   

  1. 1.河北科技大学生物科学与工程学院 2.河北省发酵工程技术研究中心
  • 收稿日期:2009-07-06 出版日期:2009-10-01 发布日期:2010-12-29
  • 通讯作者: 李 艳1

Molecular Biological Identification of Active Dry Yeast for Grape Wine

LI Yan1,2,LU Jun1,CUI Jian-dong1,2,YANG Mei-jing1   

  1. 1. College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China ;
    2. Research and Development Center for Fermentation Engineering of Hebei Province, Shijiazhuang 050018, China
  • Received:2009-07-06 Online:2009-10-01 Published:2010-12-29
  • Contact: LI Yan

摘要:

通过分子生物学方法对5种常用的葡萄酒活性干酵母进行区分鉴定。所用的分子手段为PCR-RFLP结合inter-δ PCR的方法,将活性干酵母在亚种甚至菌株水平上进行区分,得到5种活性干酵母独特的inter-δPCR扩增图谱,并且预测扩增产物的分子量。

关键词: 活性干酵母, 葡萄酒, 区分鉴定, PCR-RFLP(聚合酶链式反应-限制性片段长度多态性), inter-δPCR

Abstract:

Five commonly used active dry yeasts for grape wine were differentiated and identified by molecular biological techniques. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and inter-δPCR were used to differentiate and identify at subspecies or even strains level. Five unique inter-δPCR amplification patterns were obtained and molecular sizes of amplified products were predicted.

Key words: active dry yeasts, grape wine, differentiate and identify, PCR-RFLP, inter-δPCR

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