食品科学 ›› 2009, Vol. 30 ›› Issue (19 ): 214-217.doi: 10.7506/spkx1002-6630-200919049

• 生物工程 • 上一篇    下一篇

双歧杆菌22-5产胞外多糖发酵条件的优化

丁薛龙,沈 骞,陈 倩,尚 楠,旭日花,梁志宏   

  1. 中国农业大学食品科学与营养工程学院
  • 收稿日期:2009-08-26 出版日期:2009-10-01 发布日期:2010-12-29
  • 通讯作者: 梁志宏
  • 基金资助:

    国家大学生创新性实验计划项目(081001914);国家“863”计划项目(2008AA10Z324)

Optimizing Fermentation Conditions for High Productivity of Exopolysaccharide from Bifidobacterium 22-5

DING Xue-long,SHEN Qian,CHEN Qian,SHANG Nan,XU Ri-hua,LIANG Zhi-hong*   

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Received:2009-08-26 Online:2009-10-01 Published:2010-12-29
  • Contact: LIANG Zhi-hong

摘要:

在5L发酵罐中对双歧杆菌22-5产胞外多糖的发酵条件进行研究。针对发酵液pH值、培养温度和接种量3个主要影响因素,采用L9(34)正交试验对双歧杆菌22-5高产胞外多糖条件进行优化,优化结果为发酵液恒定pH值为7.0,培养温度为31℃,接种量为3.0%。在此优化条件下,胞外多糖的产量达到了5.8g/L,是优化前的1.75倍。

关键词: 双歧杆菌, 胞外多糖, 正交试验

Abstract:

In order to study optimal conditions for high productivity of exopolysaccharide (EPS), fermentation experiments of Bifidobacterium 22-5 were conducted through orthogonal design based on three factors: pH of fermentation medium, temperature of fermentation medium and inoculation amount. Results indicated that optimal fermentation conditions for high productivity of EPS from Bifidobacterium 22-5 were stable pH at 7.0 for fermentation broth, 31 ℃ as the fermentation temperature and 3.0% inoculation amount. Under these optimal conditions, the productivity of EPS reached 5.8 g/L with 1.75-fold enhancement.

Key words: Bifidobacterium, exopolysaccharide, orthogonal design

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