食品科学 ›› 2004, Vol. 25 ›› Issue (11): 233-234.

• 分析检测 • 上一篇    下一篇

树头发的氨基酸含量分析

 蒋盛岩, 张前军, 陈立德, 赵良忠, 段林东, 王放银   

  1. 邵阳学院生物工程系,南华大学
  • 出版日期:2005-11-15 发布日期:2011-10-24

Analysis of Amino Acids of Pterula umbrinella Bres.

 JIANG  Sheng-Yan, ZHANG  Qian-Jun, CHEN  Li-De, ZHAO  Liang-Zhong, DUAN  Lin-Dong, WANG  Fang-Yin   

  1. 1.Bioengineering Department,Shaoyang College;2. Nanhua University
  • Online:2005-11-15 Published:2011-10-24

摘要: 采用异硫氰酸苯酯(PITC)柱前衍生,反相高效液相色谱法即PICO-TAG方法对树头发的氨基酸含量进行测定,得出树头发的氨基酸色谱图。结果表明:除色氨酸被水解破坏而未检出外,树头发具有16种氨基酸,9种必需氨基酸,其氨基酸的总含量达179.652μg/mg,总必需氨基酸含量为63.546μg/mg,必需氨基酸含量占总含量的35.37%,该测定结果为树头发的药用开发提供参考。

关键词: 树头发, 氨基酸, 反相高效液相色谱法, PICO-TAG方法

Abstract: The analysis of amino acids in Pterula umbrinella Bres. was made with reversed-phase high performance liquidchromatography,using phenylisothiocyanate as a precolum derivatizing agent(i.e.pico-Tag method).The results showed that kindsof amino acid(included kinds of essential amino acids)existed in Pterula umbrinella Bres.,except that tryptophane destroyed byhydrolysis is not checked out. The sum of amino acids was 179.652μg/mg. The sum of essential amino acids was 63.546μg/mg.The percentage of essential amino acids was 35.37%.The results provide reference for exploitation of Pterula umbrinella Bres.resources.

Key words: Pterula umbrinella Bres, amino acids, reversed-phase high performance liquid chromatography, PICO-TAG