关键词: 转基因食品, 多重PCR, 聚合酶链反应, 扩增图谱

Abstract: Multiplex PCR procedures were developed for simultaneously detection multiple target sequences in geneticallymodified soybean. In order to eliminate any false negatives results, lectin and β-actin genes in soybean were included asinternal control targets to assess the efficiency of all reactions. Reliable identification of genetically modified soybean was stillachieved when its content was 0.15%, indicating high levels of sensitivity. The systems described herein is one of simple,accurate and sensitive detection methods in which only one reaction was necessary to detect multiple genetically modified targetsequences that could be reliably used for routine analysis of raw and processed food production.

Key words: genetically modified food, multiplex PCR, polymerase chain reaction, amplication profile