食品科学 ›› 2009, Vol. 30 ›› Issue (20 ): 143-145.doi: 10.7506/spkx1002-6300-200920027

• 工艺技术 • 上一篇    下一篇

波纹巴非蛤生物活性肽的分离与提纯研究

陈纯馨,陈 忻,岑佩强,陈晓刚   

  1. 佛山科学技术学院
  • 收稿日期:2009-07-20 出版日期:2009-10-15 发布日期:2010-12-29
  • 通讯作者: 陈纯馨, E-mail:Fschenxin@tom.com

Isolation and Purification of Bioactive Peptides from Paphia undulate Meat

CHEN Chun-xin,CHEN Xin,CEN Pei-qiang,CHEN Xiao-gang   

  1. Foshan Academy of Science and Technology, Foshan 528000, China
  • Received:2009-07-20 Online:2009-10-15 Published:2010-12-29
  • Contact: CHEN Chun-xin E-mail:Fschenxin@tom.com

摘要:

以波纹巴非蛤为原料,根据最佳酶解条件制备酶解液。通过凝胶层析柱(Sephadex G-25)分离水解所得粗品波纹巴非蛤酶解液,并用紫外可见分光光度计测定收集后的各管溶液,然后根据各吸光度值绘制的管数-吸光度曲线图得出第三个峰附近的管数为小分子段。根据标准曲线计算出活性肽的分子量为753.58D,最后通过反相高效液相色谱-质谱联用技术测出活性肽的分子量为679.4D。

关键词: 波纹巴非蛤, 凝胶层析, 活性肽

Abstract:

Clam (Paphia undulata) meat was enzymolyzed and the hydolysate obtained was separated into 3 fractions by Sephadex G-25 column chromatography. The principle of gel filtration chromatography demonstrated that the last fraction eluted from Sephadex G-25 had low molecular weight, which was estimated to be 753.58 D by Sephadex G-25 column chromatography and 679.4 D by RP-HPLC-MS.

Key words: Paphia undulata, gel chromatography, active peptides

中图分类号: