食品科学 ›› 2003, Vol. 24 ›› Issue (12): 146-149.

• 专题论述 • 上一篇    下一篇

蔬菜中脂氧合酶活性的快速测定

 生吉萍, 刘开朗, 申琳   

  1. 中国农业大学食品学院
  • 出版日期:2003-12-15 发布日期:2011-12-13

Rapid Methods for Lipoxygenase Assay in Vegetables

 SHENG  Ji-Ping, LIU  Kai-Lang, SHEN  Lin   

  1. College of Food Science, China Agricultural University
  • Online:2003-12-15 Published:2011-12-13

摘要: 本文综述了蔬菜中脂氧合酶活性的快速测定方法,即甲基蓝染色(MBB)、胡萝卜染色(CB)和淀粉-碘化钾(KI-S)法,通过与常规实验室测定方法和感官评价比较,发现以上方法快速而有效,可作为食品生产中行之有效的半定量检测方法,但对于不同的蔬菜种类可采用不同的快速测定方法。

关键词: 脂氧合酶, 蔬菜, 快速测定, 亚甲蓝, 胡萝卜染色, 淀粉-碘化钾

Abstract: The potential rapid methods for lipoxygenase assay in vegetables were reviewed in this paper, which includedmethylene blue bleaching (MBB), carotene bleaching (CB) and potassium iodide-starch (KI-S) methods. These methods wereevaluated both spectrophotometrically and visually. The results showed that MBB, KI-S and CB were effective for half-quantitive assay, but different for different vegetables.

Key words: lipoxygenase, vegetable, rapid methods, methylene blue bleaching, carotene bleaching, potassium iodide-starch