关键词: 毛细管电泳, 枣, 蔗糖, 葡萄糖, 果糖

Abstract: As main ingredients, sucrose, glucose and fructose in jujube could be simultaneously determined by capillaryelectrophoresis with electrochemical detection. The three species could be well separated within 15min under optimizedconditions. The effects of detection potential, concentration of electrolyte, separation voltage and injection time on the analyticalcharacterization were investigated. A copper electrode was used as the working electrode with the detection potential of +0.75V(vs. Ag/AgCl). The solutions of 0.1 mol/L NaOH were chosen as the electrophoretic media. Good linear relationship betweenpeak current and concentration existed in the range of 0.002～2 mmol/L for the three analytes studied. Good sensitivity andprecision was obtained under the optimized conditions with the detection limits of 0.78、0.46、0.71μmol/L(3 times S/N)andpeak height RSDs(n=7,c=0.4mmol/L) of 2.13%,1.22%,0.26% for sucrose, glucose and fructose respectively.

Key words: capillary eletrophoresis, jujube, sucrose, glucose, fructose