食品科学 ›› 2003, Vol. 24 ›› Issue (12): 89-91.

• 分析检测 • 上一篇    下一篇

反相高效液相色谱法测定发酵液中乳酸的含量

 郑志, 姜绍通, 潘丽军, 黄静   

  1. 合肥工业大学生物与食品工程学院
  • 出版日期:2003-12-15 发布日期:2011-12-13

Study on Determination of Lactc Acid Content in Fermentertion Broth by RP-HPLC

 ZHENG  Zhi, JIANG  Shao-Tong, PAN  Li-Jun, HUANG  Jing   

  1. College of Biotechnology and Food Engineering, Hefei University of Technology
  • Online:2003-12-15 Published:2011-12-13

摘要: 本文采用反相高效液相色谱法实现了对发酵液中可能存在的5种有机酸(乳酸、乙酸、苹果酸、琥珀酸、富马酸)的分离并对乳酸定量分析效果进行研究。结果表明该方法对乳酸含量的测定具有快速,简便、灵敏、重现性好的特点,可用于发酵企业的质量控制与产品检测。

关键词: 发酵液, 乳酸, 反相HPLC

Abstract: A reversed-phase high performance liquid chromatographic (RP-HPLC) method was developed for the separationof 5 organic acids and quantitative determination of lactic acid in fermented broth. The results showed that this method wasaccurate, simple, rapid and reproducible.

Key words: fermentation broth, lactic acid, RP-HPLC