食品科学 ›› 2003, Vol. 24 ›› Issue (12): 44-46.

• 基础研究 • 上一篇    下一篇

茄子多酚氧化酶的酶学特性研究

 余华, 周洪波, 何龙海, 袁永成, 邹善智   

  1. 成都大学生物工程系,四川省出入境检验检疫局
  • 出版日期:2003-12-15 发布日期:2011-12-13

Study on Enzymatic Characteristics of Polyphenol Oxidase of Japan

 YU  Hua, ZHOU  Hong-Bo, HE  Long-Hai, YUAN  Yong-Cheng, ZOU  Shan-Zhi   

  1. 1.Department of Bioengineering, Chengdu University;2.Sichuang Province Extry-exitInspection & Quarantine Bureau
  • Online:2003-12-15 Published:2011-12-13

摘要: 本文对日本千年2号茄子中多酚氧化酶的酶学特性进行了较详细研究。结果表明:茄子中多酚氧化酶的最适pH为6.8,当体系pH≤5.0或者pH≥7.4时,能有效降低PPO活性。在30~50℃下PPO具有较高活性,其最适温度为30℃左右。PPO的热稳定性较差,沸水加热1min时,酶活已丧失87.57%。柠檬酸、抗坏血酸、亚硫酸钠、亚硫酸氢钠、EDTA-2Na等对PPO活性有抑制作用,其中抗坏血酸抑制作用最强。

关键词: 茄子, 多酚氧化酶, 酶活性, 影响因素

Abstract: This paper made a through study for many characteristics of polyphenol oxidase of Japan Qiannian number twoeggplant.The results showed that its optimum pH was 6.8,and the optimum temperature was around 30℃.It had less heatstability,and lost 87.57% when it had been heated at 100℃ for 1min.VC,citric acid,NaHSO3,Na2SO3,EDTA-2Na and other colorpreserving agents had inhibiting effect on the activity of polyphenol oxidase,where VC was the most effective inhibitor.

Key words: eggplant, polyphenol oxidase, activity of enzyme, affect factors