条斑紫菜多糖的分离纯化及Mark-Houwink方程参数测定

食品科学 ›› 2006, Vol. 27 ›› Issue (9): 41-44.

条斑紫菜多糖的分离纯化及Mark-Houwink方程参数测定

周存山, 马海乐, 曾慧琴

江苏大学生物与环境工程学院；江苏省农产品生物加工与分离工程技术研究中心

出版日期:2006-09-15 发布日期:2011-10-20

Purification and Determination of Mark-Houwink Equation Constants of Porphyra yezoensis Polysaccharides

ZHOU Cun-Shan, MA Hai-Le, ZENG Hui-Qin

1.School of Biological and Environmental Engineering, Jiangsu University, Zhenjiang 212013, China; 2.Jiangsu Province Research Center of Bio-process and Separation of Agri-products, Zhenjiang 212013, China

Online:2006-09-15 Published:2011-10-20

摘要/Abstract

摘要： 目的:获得均一性的条斑紫菜多糖,测定相应的Mark-Houwink方程参数。方法:条斑紫菜经超临界CO2脱脂、水提、醇沉、脱蛋白和DEAE-52柱层析分级分离,获得条斑紫菜多糖,凝胶渗透色谱法鉴定多糖的均一性并同时测定分子量,利用均一性的条斑紫菜多糖测定其Mark-Houwink方程参数,非均一性的条斑紫菜多糖进行验证。结果:分离到分子量分别为2.2×105、3.8×105和6.1×105的均一性条斑紫菜多糖的组分,确定了条斑紫菜多糖在水溶液中25℃下的Mark-Houwink方程的参数为:K=7.86×10-3,α=0.626。结论:经检验,测定的K和α的值可靠,可用于条斑紫菜多糖分子量的研究。

关键词: 条斑紫菜多糖, 分离纯化, Mark-Houwink方程

Abstract: Objective: To obtain homogeneous Porphyra yezoensis polysaccharides (PYPS) and determine corresponding Mark- Houwink constants. Method: PYPS were obtained as follows: ground and degreased by super critical fluid CO2, extracted in distilled water, and then precipitated by ethanol and purified by way of removing protein with chromatography on DEAE-52 column. Molecular weight and purity were identified by GPC. The Mark-Houwink equation constants were determined by 3 homogeneous PYPS, and validated. Results: Three purified PYPS are obtained with the molecular weight as 2.2×105, 3.8×105 and 6.1×105, respectively. The Mark-Houwink equation constants of PYPS in distilled water at 25℃ are: α=0.626 and K=7.86×10-3. Conclusion: The obtained K and α value are reliable, was confirmed by PYPS2 assays. The values could be applied in molecular weight studies on PYPS molecular weights.

Key words: , Porphyra yezoensis polysaccharides； purification； Mark-Houwink equation；

周存山, 马海乐, 曾慧琴. 条斑紫菜多糖的分离纯化及Mark-Houwink方程参数测定[J]. 食品科学, 2006, 27(9): 41-44.

ZHOU Cun-Shan, MA Hai-Le, ZENG Hui-Qin. Purification and Determination of Mark-Houwink Equation Constants of Porphyra yezoensis Polysaccharides[J]. FOOD SCIENCE, 2006, 27(9): 41-44.

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