食品科学 ›› 2006, Vol. 27 ›› Issue (9): 84-87.

• 基础研究 • 上一篇    下一篇

枇杷核黄酮类物质微波法提取及对羟自由基清除作用研究

 焦士蓉,  王玲   

  1. 四川大学公共卫生学院; 西华大学理化学院;西华大学生物工程学院
  • 出版日期:2006-09-15 发布日期:2011-10-20

Study on Flavonoids Extracted from Loquat Pit by MAE and Hydroxyl Radical Scavenging Effects

 JIAO  Shi-Rong,   Wang-Ling   

  1. 1.School of Public Health,Sichuan University, Chengdu 610041, China; 2.School of Bioengineering, Xihua University, Chengdu 610039, China; 3.School of Physical and Chemical, Xihua University, Chengdu 610039, China
  • Online:2006-09-15 Published:2011-10-20

摘要: 本文对微波法提取枇杷核中黄酮类物质进行了研究,通过单因素及L9(34)正交试验,得到最佳工艺条件:m(乙醇溶液)/m(枇杷核)=15,提取功率为296W,时间120s提取3次,50%的乙醇溶液。按此最佳方法总黄酮提取率为1.41%。微波法与传统溶剂提取法比较,具有提取时间短、提取率高等优点。同时研究了该提取物对羟自由基清除作用,黄酮类物质对羟自由基的清除作用呈线性相关,相关系数R2为0.9865。

关键词: 枇杷核, 微波提取, 黄酮类化合物, 羟自由基, 清除

Abstract: The paper studied the extracting of flavonoids in loquat pit with microwave assisted extraction (MAE). The single factor and orthogonal design methods L9(34) were applied for the optimal technological conditions: m(ethanol)/m(loquat pit)=15, microwave power 296W and 3 times extracting with microwave for 120 seconds, and 50% ethanol. In these conditions, the yield of flavonoids is 1.41%. As compared with tradition ethanol extraction, the microwave extraction is a rapid method with higher yield. At the same time, a linear relationship exists between scavenging of hydroxyl radical and the extracting of flavonoids in loquat pit (R2=0.9865).

Key words: loquat pit, microwave assisted extraction (MAE), flavonoids, hydroxyl radical, scavenging