食品科学 ›› 2008, Vol. 29 ›› Issue (10): 59-62.

• 基础研究 • 上一篇    下一篇

紫甘薯花色苷色素抑制金黄色葡萄球菌作用初探

 韩永斌, 朱洪梅, 顾振新, 范龚健, 吕莹爽   

  1. 南京农业大学农业部农畜产品加工与质量控制重点开放实验室; 山西师范大学食品科技学院;
  • 出版日期:2008-10-15 发布日期:2011-12-08

Preliminary Study on Inhibitory Effect of Anthocyanins from Purple Sweet Potato Against Staph.aureus

 HAN  Yong-Bin, ZHU  Hong-Mei, GU  Zhen-Xin, FAN  Gong-Jian, 吕Ying-Shuang   

  1. 1.Key Laboratory of Food Processing and Quality Control,Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095,China;2.College of Food Science and Technology,Shanxi Normal University,Linfen 041004,China
  • Online:2008-10-15 Published:2011-12-08

摘要: 本实验研究紫甘薯花色苷色素对金黄色葡萄球菌的抑制作用。结果表明,紫甘薯花色苷色素对金黄色葡萄球菌抑制的最低浓度为200μg/ml。金黄色葡萄球菌生长曲线和透射电镜观察表明,紫甘薯花色苷的抑菌作用可能是通过增强细胞膜的通透性,使细胞异常生长,抑制对数生长期的细胞分裂,使细胞质稀薄、解体,导致细胞死亡。SDS-PAGE分析表明,紫甘薯花色苷对大肠杆菌和金黄色葡萄球菌蛋白表达影响不明显,未见特征性条带的消失,仅对部分蛋白质合成量有影响。

关键词: 紫甘薯花色苷色素, 金黄色葡萄球菌, 抑菌作用

Abstract: The reults of bacteriostatic experiment of anthocyanins from purple sweet potato indicated that their minimum inhibitory concentration (MIC) on Staph.aureus is 200μg/ml. The observation under transmission electron microscope (TEM) was carried out and the growth curve of Staph.aureus was analyzed. It was indicated that the anthocyanins might change the permeability of cell membrane and make cell excrescent to inhibit cell division. As a result,cytoplasm becomes thin,disas-sembles and even dies. SDS-PAGE analysis suggested that the anthocyanins have some effects on synthesized quantities of some proteins,but no disappearence of special protein lines is found.

Key words: anthocyanins from purple sweet potato, Staph.aureus, inhibitory effect