食品科学 ›› 2006, Vol. 27 ›› Issue (9): 167-171.

• 工艺技术 • 上一篇    下一篇

婴儿配方乳蛋白水解物在酶-膜反应器中连续化生产试验研究

 陈静,  张和平,  孙健   

  1. 中国农业大学食品科学与营养工程学院; 内蒙古农业大学乳品生物技术与工程教育部重点实验室; 蒙牛乳业(北京)有限责任公司
  • 出版日期:2006-09-15 发布日期:2011-10-20

Study on Infant Formulas in a Continuous Stirred Tank Enzyme-membrane Reactor

 CHEN  Jing,   Zhang-He-Ping,   Sun-Jian   

  1. 1.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 2. Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Huhht 010018, China;3.Inner Mongolia Mengniu Diary Corporation, Beijing 101107, China
  • Online:2006-09-15 Published:2011-10-20

摘要: 本试验首先通过调整牛乳中酪蛋白与乳清蛋白的比例为40:60,然后使其在连续搅拌式酶-膜反应器(continuousstirredtankmembranereactor,CSTMR)中水解以生产婴儿配方乳的基料。通过对三种蛋白水解酶的比较筛选最终确定以胰蛋白酶为水解用酶;单因素试验确定模拟母乳在CSTMR中水解的最适温度40℃、最适pH8.0;通过正交试验得出乳蛋白在CSTMR中水解的最适酶底比(E/S,W/W)1.84%、最适底物浓度(S,W/V)4%、最适压力(P,MPa)为0.05MPa;对乳蛋白水解物做SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)检测,发现乳蛋白水解液中酪蛋白的谱带较水解之前明显减轻,电泳凝胶扫描分析结果显示,酪蛋白中的αs-酪蛋白几乎被水解掉90%,乳清中的β-乳球蛋白也有50%以上的降解。

关键词: 婴儿配方乳, 酶-膜反应器, 水解, 蛋白质, 胰蛋白酶, 牛乳

Abstract: Milk protein from cow milk was continuously hydrolyzed in an enzyme membrane reactor to produce infant formulas. Trypsin was used as the hydrolyzing enzyme to modify the protein. Optimum parameters during the CSTMR’s operation were temperature 45℃, pH8.0, enzyme substrate ratio 1.84%, substrate concentration 0.4%, and pressure 0.05MPa. Through SDS- PAGE analysis, it was found that the hydrolysate contains less proteins than the unhydrolysate, especially the αs- casein is 90% hydrolyzed and over 50% β-lactoglobulin from milk serum is degraded.

Key words: infant formulas, CSTMR(continuous stirred tank membrane reactor), hydrolyze, protein, tyrpsin, milk