食品科学 ›› 2008, Vol. 29 ›› Issue (10): 174-177.

• 工艺技术 • 上一篇    下一篇

高性能淀粉基酶固定化载体的制备研究

 罗发兴, 杨景峰, 罗志刚   

  1. 华南理工大学轻工与食品学院;
  • 出版日期:2008-10-15 发布日期:2011-12-08

Preparation of High-powered Cassava Starch-based Carrier for Immobilization of Enzyme

 LUO  Fa-Xing, YANG  Jing-Feng, LUO  Zhi-Gang   

  1. College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,China
  • Online:2008-10-15 Published:2011-12-08

摘要: 本实验采用生物酶法对淀粉进行处理,然后再用高碘酸钠对酶处理淀粉进行氧化,制备出了高性能的淀粉基酶固定化载体。对淀粉水解率、反应时间、温度、pH、高碘酸钠的浓度和高碘酸钠和淀粉的摩尔比等对载体性能的影响进行了研究。制备出的载体具有较强的吸附性能和大量的醛基,对酶具有较强的固定化能力。

关键词: 淀粉, 载体, 酶固定化

Abstract: Starch was treated by a mixture of glucoamylase and amylase,and then was oxidized by sodium metaperiodate,to prepare high-powered strach-based carrier for immobilization of enzyme. The effects of different parameters including degree of hydrolysis,reaction time,temperture,pH,concentration of sodium metaperiodate and molar ratio of sodium metaperiodate to starch on the capability of the carrier were studied. The carrier has powerful capability of immobilizing of enzyme because of its powerful adsorption ability and a great amount of aldehyde groups.

Key words: starch, immobilization of enzyme, carrier