食品科学 ›› 2008, Vol. 29 ›› Issue (10): 267-269.

• 工艺技术 • 上一篇    下一篇

菜豆凝集素提取方法的研究

 李笑梅   

  1. 哈尔滨商业大学食品工程学院;
  • 出版日期:2008-10-15 发布日期:2011-12-08

Study on Extraction Mothod of Phaseolus vulgaris Agglutinin

 LI  Xiao-Mei   

  1. College of Food Engineering,Harbin University of Commerce,Harbin 150076,China
  • Online:2008-10-15 Published:2011-12-08

摘要: 以菜豆为原料,对菜豆植物凝集素的提取工艺条件进行研究。考察提取剂、料液比、超声时间、超声功率对凝集素提取效果的影响,讨论血凝培养时间、离心转速对血凝效果及评价指标干重量的影响。结果表明:提取剂为生理盐水,正交试验优化最佳提取工艺条件为料液比1:1.0、超声时间30min、超声功率350W,此外血凝培养时间3h,分离血凝细胞的离心转速3000r/min。

关键词: 菜豆凝集素, 提取, 凝集效果, 干重量

Abstract: The factors such as extraction agent,liquid ratio,ultrasonic time,and ultrasonic power affecting on the agglutinin extraction from Phaseolus vulgaris were investigated. The effects of hemagglutination culture time and the centrifugal speed on hemagglutination effect and dry weight were also discussed. The results showed that saline as the extraction agent,the optimal extraction conditions determined by orthogonal test are: material-liquid ratio 1: 10,ultrasonic time 30 min,and ultrasonic power 350 W. The hemagglutination culture time and centrifugal speed are 3 h and 3000 r/min,respectively.

Key words: Phaseolus vulgaris agglutinin, extraction, hemagglutination effect, dry weight