食品科学 ›› 2006, Vol. 27 ›› Issue (9): 223-226.

• 分析检测 • 上一篇    下一篇

蓝莓酒中11种酚酸的高效液相色谱测定

 翁芳华,  陈建业,  温鹏飞,  黄卫东   

  1. 中国农业大学食品科学与营养工程学院葡萄与葡萄酒研究中心
  • 出版日期:2006-09-15 发布日期:2011-10-20

Detection of 11 Phenolic Acids of Blueberry Wine by High Performance Liquid Chromatography

 WENG  Fang-Hua,   Chen-Jian-Ye,   Wen-Peng-Fei,   Huang-Wei-Dong   

  1. Centre for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Online:2006-09-15 Published:2011-10-20

摘要: 实验采用LiChrospher100RP-18e色谱柱(250×4.0mmID,5μm),以甲醇:乙酸:水溶液为流动相,流速为1.0ml/min梯度洗脱,SPD-6AV紫外检测器,30℃柱温,紫外检测波长为280nm。建立了一种新的同时测定蓝莓酒中11种酚酸含量的高效液相色谱法,并测定了蓝莓酒发酵样中酚酸的含量。

关键词: 高效液相色谱, 蓝莓酒, 酚酸

Abstract: It’s a new method for simultaneously determination of 11 phenolic acids in blueberry wines by high performance liquid chromatography was studied. Chromatographic separation was performed with LiChrospher 100RP-18e(250×4.0mmID,5μ m) by using the mobile phase gradient elution of methanol-acetic acid mixture at 30℃, flow rate at 1.0ml/min and detection wavelength at 280nm. It was successful to separate the 11 phenolic acids with a high recoveries of 11 phenolic acids (>89%), also a lower detection limit. The content of 11 phenolic acids in blueberry wines of different manufactures were also determined.

Key words:  , high performance liquid chromatography(HPLC); blueberry wine; phenolic acid;