微胶囊化儿茶素对阿霉素肾病大鼠Podocin表达的影响

食品科学 ›› 2006, Vol. 27 ›› Issue (9): 236-241.

微胶囊化儿茶素对阿霉素肾病大鼠Podocin表达的影响

何小解, 易著文, 白海涛, 莫双红, 张慧琼, 耿文茂, 党西强

中南大学小儿肾脏病研究室湖南省小儿肾脏病临床中心

出版日期:2006-09-15 发布日期:2011-10-20

Catechin Microcapsule on Podocin Expression in Rats with Nephrotic Syndrome Induced-adriblastine

HE Xiao-Jie, Yi-Zhu-Wen, Bai-Hai-Tao, MO Shuang-Hong, Zhang-Hui-Qiong, Geng-Wen-Mao, Dang-Xi-Qiang

Laboratory of Pediatric Nephrology, The Institute of Pediatrics, The Second Xiangya Hospital of Central South University, Hunan Province Clinic Central of Pediatric Nephrology, Changsha 410011, China

Online:2006-09-15 Published:2011-10-20

摘要/Abstract

摘要： 目的:观察微胶囊化儿茶素对阿霉素肾病大鼠足细胞Podocin蛋白及基因表达的影响。方法:将120只雌性SD大鼠随机分为对照组、肾病组、激素组、VE组、儿茶素组和微胶囊组共六组,尾静脉一次性注射阿霉素(5mg/kg)制备肾病模型;实验第四周末和第六周末杀鼠取血、肾组织,免疫荧光法检测肾组织中Podocin的表达;同时利用RT-PCR检测肾组织中Podocin基因表达。结果:实验第四周末,各组大鼠足细胞Podocin蛋白表达均低于对照组(p<0.01)。实验第六周末,激素组大鼠Podocin蛋白表达与对照组无统计学差异,其它各组之间相比,儿茶素组与微胶囊组Podocin蛋白表达高于VE组(p<0.01),微胶囊组高于儿茶素组(p<0.05),PodocinmRNA表达在实验末与蛋白表达一致。结论:微胶囊化儿茶素可上调阿霉素肾病大鼠肾组织中Podocin蛋白与基因表达,微胶囊技术有助于提升儿茶素的生物学效应。

关键词: 微胶囊, 儿茶素, 肾病变综合征, WT1, 大鼠

Abstract:
【Abstract】 Objective: To observe the effects of catechin microcapsulation on WT1 expression in rats with nephrotic syndrome induced-adriblastine. Methods: 112 female SD rat are randomly distributed respectively in control group, nephrotic syndrome group, dexamethason group, VE group, catechin group and microcapsule group. Catechin microcapsule is made by the way of mist spray dehydration and nephrotic syndrome rats are caused by injecting adriblastine. Before rats killed, we collect blood and kidney tissue, Podocin expression in kidney is observed by indirect immunofluorescence. Podocin mRNA expression in kidney is observed with RT-PCR. Results: Expressions of Podocin on foot process in the other groups are all lower than that of the control, while by the the 6th, there is no significant difference between that of the Dexamethason and the control, and it was higher in the catechin group and the catechin microcapsulation -treated group compared with that of the VE group. When the former two groups were compared, that in catechin microcapsulation -treated group was higher than that in catechin -treated group. Podocin mRNA expression were accord with Podocin protein expression. Conclusion: Catechin microcapsulation could up-regulate Podocin expression of protein and gene in rats with nephrotic syndrome induced-adriblastine, the technology of microencapusulation could help to upgrade the biologic effect of catechin.

Key words: microcapsulation, catechin, nephrotic syndrome, Podocin, rat

何小解, 易著文, 白海涛, 莫双红, 张慧琼, 耿文茂, 党西强. 微胶囊化儿茶素对阿霉素肾病大鼠Podocin表达的影响[J]. 食品科学, 2006, 27(9): 236-241.

HE Xiao-Jie, Yi-Zhu-Wen, Bai-Hai-Tao, MO Shuang-Hong, Zhang-Hui-Qiong, Geng-Wen-Mao, Dang-Xi-Qiang. Catechin Microcapsule on Podocin Expression in Rats with Nephrotic Syndrome Induced-adriblastine[J]. FOOD SCIENCE, 2006, 27(9): 236-241.

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