食品科学 ›› 2003, Vol. 24 ›› Issue (10): 131-135.

• 分析检测 • 上一篇    下一篇

选择性增菌液对单核细胞增生李斯特氏菌的影响

 卢红梅, 张义明   

  1. 贵州工业大学化学与生物工程学院
  • 出版日期:2003-10-15 发布日期:2011-12-13

Effects Study on Selective Enrichment Broth Incubated from Listeria Monocytogenes

 LU  Hong-Mei, ZHANG  Yi-Ming   

  1. College of Chemical and Biological Engineering,Guizhou University of Technology
  • Online:2003-10-15 Published:2011-12-13

摘要: 将单核细胞增生李斯特氏菌(Listeria monocytogenes)不同温度下培养在非选择性培养基TSBYE和选择性培养基UVM-LEB、DFB和FB中,从菌体的生长曲线、最高生长温度和最大生长量反映出选择性增菌液对单核细胞增生李斯特氏菌的生长有较明显的抑制作用,其中FB的抑制程度较大,UVM-LEB和DFB的抑制程度较小且相当;37℃下在UVM-LEB、DFB和FB中培养24h能检出的最低菌体浓度分别为5.2个/ml 、52个/ml和5200个/ml,而培养48h相应的值为0.52个/ml、 0.052个/ml和0.52个/ml;比较而言,DFB和FB更优于UVM-LEB。

关键词: 单核细胞增生李斯特氏菌, 选择性增菌液, 非选择性增菌液

Abstract: L. monocytogenes was incubated into non-selective enrichment broth TSBYE and selective enrichment broth UVM-LEB, DFB and FB with different temperatures. Comparing the growth curve, the highest growth temperature and the bestgrowth amount in TSBYE, UVM-LEB, DFB and FB were obtained. The growth of L. monocytogenes was evidently inhibitedby the selective media and the inhibition of FB was better than UVM-LEB and DFB,while the inhibition of DFB almost equaledto UVM-LEB. The lowest measurable concentrations of L. monocytogenes in UVM-LEB, DFB and FB were 5.2/ml 、52/mland 5200/ml respectively after 24 hours incubation,and 0.52/ml、0.052/ml and 0.52/ml respectively after 48 hours incubation.As selective enrichment broth, DFB and FB were better than UVM-LEB.

Key words: Listeria monocytogenes, selective enrichment broth, non-selective enrichment broth