食品科学 ›› 2003, Vol. 24 ›› Issue (9): 119-121.

• 分析检测 • 上一篇    下一篇

溶菌酶活力测定方法的改进

 谢红伟   

  1. 贵州大学
  • 出版日期:2003-09-15 发布日期:2011-12-13

Improvement of Lysozyme Activity Measurement

 XIE  Hong-Wei   

  • Online:2003-09-15 Published:2011-12-13

摘要: 本文以壳聚糖代替溶壁微球菌作底物测定溶菌酶的活力。实验证明:在55℃,pH=4.5时,达到最大酶反应速度。酶浓度在0~4.5U/ml范围内符合郎伯—比尔定律,回归方程为Y=0.2173X-0.0114,线性相关系数为γ=0.9998。

关键词: 溶菌酶, 壳聚糖, 酶活力, 溶壁微球菌

Abstract: Chitosan was used to replace the substrate M.Lysodeikticus to determine the enzyme activity of lysozyme.It showedthat the optimum temperature and optimum pH were 55℃ and 4.5 respectively and it was accordant with the lamber-bier lawwhen the enzyme concentration was 0~4.5U/ml.The regression equation is Y=0.2173X-0.0114 with linear correlationcoefficient as 0.9989.

Key words: chitosan, lysozyme, enzyme activity, M.Lysodeikticus