关键词: 蜂王浆, 氟胺氰菊酯, 氟氯苯氰菊酯, 超高效液相色谱

Abstract:

In this article, a study on extraction, clean-up and ultra-performance liquid chromatographic analysis was carried out. The samples were dissolved using water and extracted by dichloromethane / hexane (1:1，V/V). The sample emulsification was removed by absolute ethanol and was cleaned up by reverse-phase column, while the two residues were eluted off via SPE cartridge with acetonitrile and evaporate to dryness on nitrogen dry evaporator, and residues were dissolved in 80% acetonitrile (acetonitrile/water, 80:20，V/V) and passed through 0.22 μm filter for determination by a ultra-performance liquid chromatography UV detection. A XTerra Shield RP18 column(2.1mm×50mm，1.7μm)was as chromatographic separation column, water and acetonitrile were as mobile phase under gradient elution. The total analysis time was less than 4 min. The recoveries is 75.7%～82.8%. The limit of detection for the method was 10μg/kg.

Key words: royal jelly, fluvalinate, flumethrin, ultra-performance liquid chromatography