食品科学 ›› 2008, Vol. 29 ›› Issue (11): 130-133.

• 工艺技术 • 上一篇    下一篇

碱性蛋白酶水解玉米蛋白工艺条件的研究

 刘振春, 董源, 王朝辉   

  1. 吉林农业大学食品科学与工程学院;
  • 出版日期:2008-11-15 发布日期:2011-12-08

Study on Alkaline Protease Hydrolysis of Corn Gluten Meal

 LIU  Zhen-Chun, DONG  Yuan, WANG  Chao-Hui   

  1. College of Food Engineering,Jilin Agricultural University,Changchun 130118,China
  • Online:2008-11-15 Published:2011-12-08

摘要: 通过单因素分析,对影响碱性蛋白酶水解玉米蛋白的主要因素进行了研究,利用正交试验筛选出最佳工艺参数。结果表明,酶水解最佳条件为底物浓度3%、酶浓度(E/S)3.0%、水解时间1h、温度45℃,在pH值为9.0条件下,水解度可达38.12%。

关键词: 玉米蛋白, 碱性蛋白酶, 水解, 水解度

Abstract: The factors affecting hydrolysis of corn gluten meal by alkaline protease were studied by mono-factor analysis,and the optimum technique parameters were determined by orthogonal test.The results showed that under the optimal conditions of:concentration of substrate 3%,enzyme to substrate ratio 3.0%,temperature 45 ℃,hydrolysis time 1 h and pH 9.0,the hydrolysis of degree is 38.12%.

Key words: corn gluten meal, alkaline protease, hydrolysis, degree of hydrolysis