食品科学 ›› 2003, Vol. 24 ›› Issue (9): 44-48.

• 基础研究 • 上一篇    下一篇

枯草芽孢杆菌转酮醇酶变异株C1-B941菌种特性的初步研究

 米丽娟, 孙文敬, 谢红, 赵峰梅, 杨庆文   

  1. 山西省生物研究所
  • 出版日期:2003-09-15 发布日期:2011-12-13

Study on Characteristics of the Transkelolase Mutant Bacillus subtilis

 MI  Li-Juan, SUN  Wen-Jing, XIE  Hong, ZHAO  Feng-Mei, YANG  Qing-Wen   

  • Online:2003-09-15 Published:2011-12-13

摘要: C1-B941是一株枯草芽孢杆菌(Bacillus subtilis)的转酮醇酶(Transketolase,TKT)变异株,与亲株C1相比,其在菌体形态及代谢特性上发生了显著变化,如对戊糖不利用,对葡萄糖等利用变弱,碳源利用存在抑制,表现为芳香氨基酸合成途径缺陷,对数期细胞聚集成链,孢子形成能力下降,在发酵培养基中可以积累D-核糖,发酵液具有广泛的用途。

关键词: D-核糖产生菌, 枯草芽孢杆菌, 转酮醇酶变异菌株, 菌种特性

Abstract: C1-B941 is a transketolase mutant of Bacillus subtilis., which is differentiated remarkably with the parent strain C1in the characteristics of cell morphlogy and metabolism. The results showed that: not utilizing pentoses; or weakening thecarbohydrates such as D-glucose utilization or inhibition interaction on carbohydrates ; source there would be defects in synthesisof aromatic amino acids, chains formation during exponential cell growth; and lowering of sporulation capability; It was possibleto accumulate D-ribose in the ferment medium, so as to make the fermented liguid useful in a lorge scale.

Key words: D-ribose-producing strain, Bacillus subtilis, transketolase mutant, propertise