食品科学 ›› 2008, Vol. 29 ›› Issue (11): 228-233.

• 工艺技术 • 上一篇    下一篇

螺旋藻蛋白提取方法比较研究

 刘立闯, 胡志和, 刘彤, 贾静   

  1. 天津市食品生物技术重点实验室,天津商业大学生物技术与食品科学学院;
  • 出版日期:2008-11-15 发布日期:2011-12-08

Comparative Study on Extraction Methods of Spirulina Phycobiliprotein

 LIU  Li-Chuang, HU  Zhi-He, LIU  Tong, JIA  Jing   

  1. Tianjin Key Laboratory of Food Biotechnology,College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China
  • Online:2008-11-15 Published:2011-12-08

摘要: 采用超声波法、高压均质法和反复冻融法对螺旋藻细胞进行破碎,确定破碎条件和螺旋藻蛋白溶出率。结果表明:在各自的最佳提取条件下,超声波法得到的蛋白溶出率为71.51%,高压均质法得到的藻胆蛋白溶出率为73.87%,反复冻融法得到的蛋白溶出率为72.03%。

关键词:  , 藻胆蛋白, 超声波, 高压均质, 反复冻融, 蛋白溶出率

Abstract: Three methods(ultrasonic method,high pressure homogenization and freezing-thaw disruption) were used to disrupt Spirulina cell respectively,in order to find out the optimal cell disruption conditions to increase the dissolution rate of Spirulina phycobiliprotein(SPP).Results showed that the highest dissolution rates of the SPP are 71.25%,73.87% and 72.03%,under the optimal conditions of ultrasonic method,high pressure homogenization and freezing-thaw disruption,respectively.

Key words: Phycobiliprotein, ultrasonic, high pressure homogenization, freezing-thaw, protein dissolution rate