高速逆流色谱法分离纯化绿原酸研究

食品科学 ›› 2008, Vol. 29 ›› Issue (11): 298-299.

高速逆流色谱法分离纯化绿原酸研究

陈德经, 韩豪, 董博妮

陕西理工学院陕西省资源生物重点实验室；陕西理工学院生物科学与工程学院；

出版日期:2008-11-15 发布日期:2011-12-08

Study on Separation and Purification of Chlorogenic Acid with High-speed Countercurrent Chromatography

CHEN De-Jing, HAN Hao, DONG Bo-Ni

1.Shaanxi University of Technology,Shanxi Provincial Bio-resource Key Laboratory,Hanzhong 723000,China;2.College of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong 723000,China

Online:2008-11-15 Published:2011-12-08

摘要/Abstract

摘要： 利用高速逆流色谱技术分离纯化金银花中的绿原酸。选择正丁醇-冰乙酸-水(4:1:5,V/V)系统来分离,分离结果经高效液相(HPLC)检测纯度达到98.1%,绿原酸的得率为95%。

Abstract: High-speed countercurrent chromatography was used to separate chlorogenic acid from 70% ethanol extract of dry Lonicera japonica Thunb.with three different solvent systems.It was found experimentally that the optimal solvent system is composed of butanol,acetic acid and wate,with the ratio of 4:1:5(V/V).The purity of obtained chlorogenic acid is determined as 98.1% by high performance liquid chromatograph(HPLC),and its yield is 95%.

Key words: chlorogenic acid, high-speed countercurrent chromatography(HSCCC), seperation

陈德经, 韩豪, 董博妮. 高速逆流色谱法分离纯化绿原酸研究[J]. 食品科学, 2008, 29(11): 298-299.

CHEN De-Jing, HAN Hao, DONG Bo-Ni. Study on Separation and Purification of Chlorogenic Acid with High-speed Countercurrent Chromatography[J]. FOOD SCIENCE, 2008, 29(11): 298-299.