食品科学 ›› 2003, Vol. 24 ›› Issue (8): 132-134.

• 分析检测 • 上一篇    下一篇

实时荧光PCR定量检测加工产品中转基因玉米Mon810成分

 覃文, 曹际娟, 朱水芳   

  1. 广州出入境检验检疫局,辽宁出入境检验检疫局,国家质检总局动植物检疫实验所
  • 出版日期:2003-08-15 发布日期:2011-12-13

Identified and Quantitative Detection of the Genetically Modified Maize Mon810 Components in Processed Products

 QIN  Wen, CAO  Ji-Juan, ZHU  Shui-Fang   

  • Online:2003-08-15 Published:2011-12-13

摘要: 采用实时荧光PCR技术,建立了定量(性)鉴定检测加工产品中转基因玉米Mon810成分的方法。实验设计的可以扩增玉米自身基因和外源基因边界序列的引物和探针具有品种和品系特异性,特异性地检测出食品、饲料等加工产品中转基因玉米Mon810成分。某些检测样品不仅检出转基因玉米Mon810成分,还同时检出其它转基因玉米品系或其它转基因品种。本研究实验建立的转基因玉米Mon810品系鉴定检测方法,即可以用于加工产品中转基因成分的定量检测(检测低限为0.1%),也可以用于定性检测,或作为常规PCR定性检测后的确证实验方法。

关键词: 转基因玉米Mon810, 实时荧光定量PCR, 检测

Abstract: Study the method of quantitative/identified detection of genetically modified (GM) maize Mon810 components infoods and in feeds by real-time PCR. The primer and probe set overlapping the junction was designed and used for assay. Theresults demonstrated this method is useful for identifying the event-specific GM maize Mon810. Some samples were tested notonly the GM maize Mon810 but also another GMO components.

Key words: GM Maize mon810, real-time quantitative deteclion PCR, detection