食品科学 ›› 2008, Vol. 29 ›› Issue (12): 146-149.

• 基础研究 • 上一篇    下一篇

60Co-γ射线对粳米黏度的影响及机理分析

 邹珍友, 彭霄鹏, 李杏, 张开翔, 王芳, 王国祥, 谭媛媛, 傅俊杰   

  1. 南京农业大学生命科学实验中心-天美电镜技术合作示范实验室; 浙江大学原子核农业科学研究所;
  • 出版日期:2008-12-15 发布日期:2011-12-08

Study on Effect of 60Co-γ Radiation on Viscosity of Round-shaped Rice and Its Mechanim

ZOU  Zhen-You, PENG  Xiao-Peng, LI  Xing, ZHANG  Kai-Xiang, WANG  Fang, WANG  Guo-Xiang, TAN  Yuan-Yuan, FU  Jun-Jie   

  1. 1.Co-laboratory of Life Science Experiment Center and Techcomp Company,Nanjing Agricultural University,Nanjing 210095,China;2.Institute of Nuclear Agricultural Sciences,Zhejiang University,Hangzhou 310029,China
  • Online:2008-12-15 Published:2011-12-08

摘要: 为明确大米辐照后黏度下降的原因,将粳米粒放在不同剂量的60Co-γ射线下辐照后观察了淀粉粒的形态与结构,测量了蒸煮黏度、碱溶蛋白分子量种类以及自由基含量的变化。结果表明,随辐照加强,粳米的蒸煮黏度的数值自213cPs单调降低到21cPs;淀粉组织崩解碎裂;蛋白质碎片增多,生成了35kD以下的小蛋白片段和肽;自由基增多,ESR信号增强;大米受γ射线辐照时,其成分物质中的电子从原有轨道激发电离,生成电子未配对的自由基,与淀粉、蛋白质等反应,导致后者断裂;以及辐射的生热效应使淀粉和其它成分膨胀产生的应力使大米结构崩解,也是粳米黏度降低的部分原因。

关键词: 辐照食品, 粳米, 淀粉黏度, 自由基, ESR

Abstract: In order to make clear the causes of rice viscosity decline after 60Co-γ radiation,the morphological shape and stracture of starch granules of round-shaped rice radiated by 60Co-γ at different doses were observed with scanning electron microscope,and the alkali soluble protein segments,cooking viscosity and free radicals content were measured. The results indicated that the cooking viscosity of rice diclines straight from 213 to 21 with 60Co-γ dose inscreasing from 0 kGy to 15 kGy,the starch structure of rice are disintegrated,the proteins are cleaved into segments or peptide of 35 kD or less,and the free radicals content characterized by electron spin resonance(ESR) signal rises. 60Co-γ radiation causes excitation and ionization of electron of substance in rice from original orbit,thus free radicals that do not paire with electron are produced,and the reactions of the free radicals with starch and protein cause the cleavage of the latter. All these are the causes of rice viscosity decline induced by 60Co-γ radiation,as well as the structure disintegration of rice induced by the stress of starch and other components swelling from thermic effect of 60Co-γ radiation.

Key words: irradiation food, rice, viscosity of starch, radical, ESR