关键词:  , 苦荞麦, 过敏蛋白, 克隆, 序列分析

Abstract: The N-terminal gene of major allergenic protein in tartary buckwheat (named TBb) was amplified from the total RNA of tartary buckwheat using RT-PCR and 5’-rapid amplication of cDNA ends (5’-RACE) methods. The TBb gene sequence consisted of 1005 nucleotides and a 960bp open reading frame (ORF) which encoded a protein of 320 amino acids residues. Results of homology analysis revealed that the TBb cDNA sequence shared 90% homology with the major allergenic storage protein and legumin-like protein of common buckwheat. The deduced amino acid sequence shared 84% and 76% homology with the legumin-like 13S storage protein and the major allergenic storage protein of common buckwheat respectively. The obtain- ment of the N-terminal gene of major allergenic protein in tartary buckwheat should lay an important foundation for further study of the tartary buckwheat allergen.

Key words:  , tartary buckwheat； allergenic protein； clone； sequence analysis；