食品科学 ›› 2006, Vol. 27 ›› Issue (10): 65-68.

• 基础研究 • 上一篇    下一篇

大蒜中蒜氨酸酶的氨基酸组成分析和热力学研究

 李瑜,  许时婴   

  1. 河南农业大学食品科学技术学院; 江南大学食品学院教育部食品科学与安全重点实验室
  • 出版日期:2006-10-15 发布日期:2011-11-16

Analyzing on the Amino Acid Composition and Heat Dynamics of Garlic Alliinase

 LI  Yu,   Xu-Shi-Ying   

  1. 1.College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China; 2.School of Food Science and Technology, Southern Yangtze University, Key Lab of Food Science and Safety, Ministry of Education, Wuxi 214036, China
  • Online:2006-10-15 Published:2011-11-16

摘要: 本文对首次采用SephacrylS-200凝胶柱从新鲜大蒜中分离纯化出来的蒜氨酸酶的氨基酸组成进行了分析,并对蒜氨酸酶的催化反应表观活化能和热失活动力学进行了研究。结果表明,蒜氨酸酶中酸性氨基酸的含量比较高,其中Asp含量最多,摩尔百分含量达到15.51%;蒜氨酸酶催化反应在较低温度下即可进行且蒜氨酸酶是不耐高温的酶类。

关键词:  , 大蒜, 蒜氨酸酶, 氨基酸分析, 热力学

Abstract: In this paper amino acid composition of garlic alliinase purified with Sephacryl S-200 column was analyzed. Apparent activation energy and heat inactivity dynamics were also studied. The results showed that the content of acidic amino acids were higher and among them the content of Asp was the highest, and its mol percentage content could reach 15.51%; the alliinase catalyzed reaction could perform at lower temperature and alliinase was temperature intolerance.

Key words: garlic, alliinase, amino acid analysis, heat dynamics