食品科学 ›› 2006, Vol. 27 ›› Issue (10): 143-146.

• 基础研究 • 上一篇    下一篇

亚麻籽双液相萃取中醇相组分体外抗氧化活性研究

 李高阳, 丁霄霖   

  1. 湖南省农产品加工研究所; 江南大学食品学院
  • 出版日期:2006-10-15 发布日期:2011-11-16

In virto Antioxidant Activity of Ethanol Phase Fraction from Two-liquid-phase Extraction of Flaxseed

 LI  Gao-Yang, DING  Xiao-Lin   

  1. 1.Institute of Hunan Agricultural Product Processing, Changsha 410125, China; 2.School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China
  • Online:2006-10-15 Published:2011-11-16

摘要: 采用体外实验方法对亚麻籽双液相萃取中乙醇相的氯仿、正丁醇和水三种提取物进行抗氧化活性研究。结果表明:乙醇相三个萃取组分中,以正丁醇提取物抗亚油酸氧化能力最强,比BHT略弱。氯仿提取物次之,强于VC,水提取物最弱。在对[DPPH·]自由基的清除能力上,正丁醇提取物最强,IC50为3.1μg/ml,比芦丁(IC50=3.38μg/ml)强。水提取物略弱于BHT,氯仿提取物最弱;正丁醇提取物对[·OH]自由基的清除能力最强,IC50为18.15μg/ml,比芦丁(IC50=7.96μg/ml)弱。氯仿提取物次之,水提取物最弱;正丁醇提取物螯合Fe2+的能力最强,氯仿提取物次之,而水提取物最弱。

关键词: 亚麻籽, 双液相, 醇相, 抗氧化

Abstract: The antioxidant activity of ethanol phase fraction extracted by choloform, n-butyl alcohel and water from two-liquid- phase extraction of flaxseed was evalvated respectively. The results showed that n-butyl alcohel extracts is the strongest in inhibiting linoleic acid peroxidation, less than BHT, then chloroform extraction, and then water extraction. The capacities of scavenging [DPPH·] and [·OH] of n-butyl alcohel extracts were stronger than chloroform extracts and water extracts, extracts IC50 are 3.1μg/ml and 18.15μg/ml, rutin (IC50=3.58μg/ml and 7.96μg/ml), respectively. Capacity of scavenging [DPPH·] of chloroform extracts is weaker than water extracts, but it is stronger in scavenging [·OH] than water extracts; Fe2+ chelate activity of n-butyl alcohel extracts is strongest, then chloroform extracts, and then water extracts.

Key words:  , flaxseed; two-liquid-phase; ethanol phase; antioxidation;