食品科学 ›› 2006, Vol. 27 ›› Issue (10): 311-341.

• 工艺技术 • 上一篇    下一篇

康氏木霉降解麸皮的研究

 赵玉萍   

  1. 淮阴工学院生化系
  • 出版日期:2006-10-15 发布日期:2011-11-16

Study on Wheat Bran Degraded by Trichoderma koningii

 ZHAO  Yu-Ping   

  1. Department of Bioengineering and Chemical Engineering, Huaiyin Institute of Technology, Huaia’n 223001, China
  • Online:2006-10-15 Published:2011-11-16

摘要: 对实验室分离筛选的康氏木霉进行研究。研究和分析了滤纸酶活(FPA)测定法的最优酶解条件为:在60℃、pH4.5下酶解40min,酶与底物之比为1:2。康氏木霉的最优产酶条件为28℃下,培养4d,培养基pH为6.5,含水量为150%,接种量为5%。用同时去蛋白和淀粉的麸皮作为作用底物,麸皮的转化率可高达28.5%。

关键词: 麸皮, 纤维素酶, 酶活, 转化率

Abstract: Trichoderma koningii which had been separated was studied in this paper. The conditions of the filter paper activity (FPA) test were studied and analyzed, which were showed as follows: enzymolysis for 40 minutes on the condition of 60℃ and pH4.5, proportion of cellulase to substrate was 1:2. The optimum fermentation conditions were showed as follows: the culture medium was pH6.5, water content was 150%, the amount of inoculation was 5%, tempera- ture was 28℃, fermentation time was 4d. The transformation efficiency could reach 28.5% when the wheat bran had been removed protein and starch.

Key words: wheat bran, cellulase, cellulase activity, transformation efficiency